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Anti-inflammation properties of resveratrol in the detrusor smooth muscle of the diabetic rat

  • Urology - Original Paper
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Abstract

Purpose

In this paper, we aimed to prove that resveratrol can inhibit inflammation in the detrusor smooth muscle of diabetic rats, which may provide a new direction for diabetic cystopathy (DCP) treatment.

Methods

We induced a Sprague–Dawley (SD) rat model of type 1 diabetes by intraperitoneal injections of streptozotocin (STZ). Then, we separated the SD rats into four groups: (1) an excipient-treated control group; (2) a resveratrol-treated control group; (3) an excipient-treated streptozotocin (STZ)-injected group; and (4) a resveratrol-treated STZ-injected group. We administered the resveratrol or excipient by intragastric administration. After 12 weeks of diabetes induction, we measured the blood–sugar concentrations and bladder weights, and we took the bladder tissues of each group of rats for hematoxylin–eosin staining to observe the histological changes. We used real-time quantitative polymerase chain reaction (qPCR) and Western blotting to analyze the expression levels of tumor necrosis factor-alpha (TNF-α), nuclear factor kappa B (NF-κB), interleukin (IL)-6, and IL-1β.

Results

The bodyweights of the diabetic rats were appreciably reduced, while the bladder weights and blood–glucose concentrations were substantially increased. Oral resveratrol could not improve the changes in the bodyweights and blood–glucose concentrations, but it had a certain effect on the bladder weights. In a macroscopic evaluation, the bladder walls of the STZ-induced diabetes rats were thickened, and, from the H&E staining, we could see that the bladder tissues of the diabetic rats had inflammatory cell infiltration, edema, and the capillary congestion of the mucosa and lamina propria. After resveratrol treatment, the bladder-wall thickening was reduced, and the tissue damage and inflammation were significantly ameliorated. We could associate all these changes with markedly heightened expressions of TNF-α, IL-1β, IL-6, and NF-κB in the detrusor smooth muscle (DSM) tissues of the diabetic rats. Oral treatment with resveratrol alleviated the expressivity of the inflammatory cytokines in the DSM tissues.

Conclusions

Resveratrol treatment ameliorated the histological changes in the bladder and inhibited the expressions of DSM–tissue inflammatory factors in diabetes rats. Resveratrol may provide a new direction of research for the treatment of diabetic cystopathy.

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Acknowledgements

Supported by the educational department of Liaoning Province No. QN2019018 grant.

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Authors and Affiliations

  1. Department of Urology, Fourth Affiliated Hospital, China Medical University, 4 Chongshan East Road, Shenyang, Liaoning, China

    Feihong Xu, Huifang Du, Jun Hou, Jingxuan Liu & Ning Li

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  1. Feihong Xu
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Correspondence to Ning Li.

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Xu, F., Du, H., Hou, J. et al. Anti-inflammation properties of resveratrol in the detrusor smooth muscle of the diabetic rat. Int Urol Nephrol 54, 2833–2843 (2022). https://doi.org/10.1007/s11255-022-03334-x

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