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Establishment of animal model manifested as bladder neurogenic changes generated by bilateral pelvic nerve injury in male rats

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Abstract

Purpose

To establish a male rat model of neurogenic bladder after bilateral pelvic nerve injury (BPNI) and investigate the factors associated with onset of neurogenic bladder.

Methods

Twenty-four 8-week-old male Sprague–Dawley rats were randomly divided into three groups (n = 8 rats per group). Rats in 4-week and 8-week nerve injury group underwent BPNI, while rats in the sham group underwent a sham operation. Bladder functional analysis were performed and then bladders tissues were harvested for morphological examination and investigating the mRNA expression levels of target genes in all rats.

Results

The bladder weight significantly increased in rats following BPNI. Functional analysis revealed non-voiding contractions and decreased detrusor contractility following BPNI, manifested as elevated post-void residual and bladder capacity while reduced maximum voiding pressure and voiding efficiency. The collagen area in bladder tissue and mRNA expression levels of target genes significantly increased at 4 or 8 weeks post-BPNI except Smad3. At 4 weeks post-BPNI, expression levels of vesicular acetylcholine transporter were reduced, then returned to baseline at 8 weeks. Expression levels of tyrosine hydroxylase were reduced at both 4 and 8 weeks post-BPNI.

Conclusions

A neurogenic bladder animal model was successfully established by performing BPNI in male rats, characterized by impaired voiding function, bladder detrusor fibrosis, and reduced neurotransmitter release.

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Funding

This work was supported by the Anhui Provincial Natural Science Foundation [grant numbers: 1708085QH179].

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Authors and Affiliations

  1. Department of Urology, Provincial Hospital Affiliated to Anhui Medical University, Anhui Medical University, Hefei, 230001, Anhui, People’s Republic of China

    Qingyu Ge & Jun Xiao

  2. Clinical Skills Training Center, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, People’s Republic of China

    Meiduo Wang

  3. Department of Urology, Division of Life Sciences and Medicine, The First Affiliated Hospital of USTC, University of Science and Technology of China, Hefei, 230001, Anhui, People’s Republic of China

    Yao Lin, Congyun Xu & Zhou Shen

Authors
  1. Qingyu Ge
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  5. Jun Xiao
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Contributions

QG, JX, and ZS contributed to the study conception and design. Material preparation, data collection and analysis were performed by QG, MW, YL, and CX. The first draft of the manuscript was written by QG and ZS, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Jun Xiao or Zhou Shen.

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We declare that we have no conflict of interest.

Ethical approval

Animal experiments were conducted in accordance with institutional guidelines approved by the Animal Care and Use Committee of Anhui Medical University. The work was performed at Provincial Hospital Affiliated to Anhui Medical University.

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Ge, Q., Wang, M., Lin, Y. et al. Establishment of animal model manifested as bladder neurogenic changes generated by bilateral pelvic nerve injury in male rats. Int Urol Nephrol 53, 421–429 (2021). https://doi.org/10.1007/s11255-020-02668-8

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  • DOI: https://doi.org/10.1007/s11255-020-02668-8

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