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Use of logistic models to evaluate the response of superovulation treatment and embryo production in Santa Inês ewes

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Abstract

The study aimed to verify the influence of the FecGE mutation in superovulated ewes and to evaluate the probability of logistic models to determine the response capacity of these ewes to superovulatory treatment. Santa Inês ewes (n = 29) were genotyped for the FecGE mutation and separated for their genotype group in carriers of the mutant E allele (FecGE/E, FecG+/E) and non-carrier (FecG+/+) alleles. The ewes underwent hormonal treatment for superovulation. Aside from the genotypes, variables included in the statistical model were reproductive status (empty, early lactation, or late lactation), age (> or < 6 years), and number of births (nulliparous, primiparous, multiparous). The carriers of the mutation could be discriminated from the non-carriers based on the number of corpora lutea, rate of frozen embryos, and fecundity. Recovery rate was significantly higher (P < 0.05) in FecGE/E (94.31%) compared to FecG+/E (63.15%) and FecG+/+ (61.90%) (P < 0.05), whereas fecundity rate of FecG+/+ ewes (50.76%) was significantly higher than FecG+/E (18.96%) and FecGE/E (32.53%) (P < 0.05). We determined in this study that the response to superovulation and embryo production can be discriminated between FecGE/E and FecG+/E ewes in relation to the FecG+/+ genotype. Logistic models that included reproductive status and mutation, or reproductive status and age, or reproductive status and number of births were effective in predicting the response to superovulatory treatment.

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Funding

The study was financially supported, granted as a scholarship, by the Coordination for the Improvement of Higher Education Personnel (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—CAPES) of Brazil, Finance Code 001.

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Authors and Affiliations

  1. Reproductive Biotechniques Laboratory, Federal Rural University of Pernambuco, Street Dom Manuel de Medeiros, s/n-Dois Irmãos, Recife-PE, 52171-900, Brazil

    Maiana Silva Chaves, José Carlos Ferreira-Silva, Cláudio Coutinho Bartolomeu & Marcos Antonio Lemos de Oliveira

  2. Brazilian Agricultural Research Corporation-Embrapa Biotechnology and Genetic Resources, Parque Estação Biológica, PqEB, Avenue W5 Norte, Brasília-DF, 70770-917, Brazil

    Alexandre Floriani Ramos, Nathalia Hack Moreira Brasil, Samuel Rezende Paiva & Eduardo Oliveira Melo

  3. Federal University of Tocantins, Avenida NS-15, Quadra 109, Norte, s/n-Plano Diretor Norte, Palmas-TO, 77001-090, Brazil

    Eduardo Oliveira Melo

  4. Brazilian Agricultural Research Corporation-Embrapa National Dairy Cattle Research Center, 15, Street Eugênio do Nascimento, 610-Dom Bosco, Juiz de Fora-MG, 36038-330, Brazil

    Inácio Barros

  5. Laboratory of Physiology and Control of Reproduction, Faculty of Veterinary, State University of Ceará, Avenue Dr. Silas Munguba, Fortaleza-CE, Itaperi, 170060714-903, Brazil

    Vicente José de Figueirêdo Freitas

  6. Federal University of Sergipe, Rodovia Engenheiro Jorge Neto, km 3-Silos, Nossa Senhora da Glória-SE, 49680-000, Brazil

    Valdir Ribeiro Junior

  7. Brazilian Agricultural Research Corporation-Embrapa Tabuleiros Costeiros, Avenue Beira Mar, 17 3250-Jardins, Aracaju-SE, 49025-040, Brazil

    Hymerson Costa Azevedo

Authors
  1. Maiana Silva Chaves
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  2. Alexandre Floriani Ramos
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  3. Nathalia Hack Moreira Brasil
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  4. José Carlos Ferreira-Silva
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  11. Marcos Antonio Lemos de Oliveira
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  12. Hymerson Costa Azevedo
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Contributions

Maiana S. Chaves: conceptualization, methodology, investigation, and writing of the original draft; Alexandre F. Ramos and Nathalia H. M. Brasil: methodology and formal analysis; José C. Ferreira-Silva: support on writing; Samuel R. Paiva: ewe genotyping; Eduardo O. Melo: ewe genotyping; Inácio Barros: statistical analysis; Vicente José F. Freitas: support on the review and editing of the original draft; Valdir R. Junior: statistical analysis; Cláudio C. Bartolomeu: support on writing; Marcos A. L. Oliveira: support on the review and editing of the original draft editing of the original draft; Hymwrson C. Azevedo: supervision, support on the review, and editing of the original draft.

Corresponding author

Correspondence to Hymerson Costa Azevedo.

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This research was conducted after evaluation and approval from the Committee of Ethics in Animal Use, Brazilian Agricultural Research Company—Embrapa, Tabuleiros Costeiros Unit, Aracaju-SE, Brazil (License: 13072016.006).

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Chaves, M.S., Ramos, A.F., Brasil, N.H.M. et al. Use of logistic models to evaluate the response of superovulation treatment and embryo production in Santa Inês ewes. Trop Anim Health Prod 54, 276 (2022). https://doi.org/10.1007/s11250-022-03310-8

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