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Interactive effect of amino acids balanced at ideal lysine ratio and exogenous protease supplemented to low CP diet on growth performance, carcass traits, gut morphology, and serum metabolites in broiler chicken

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Abstract

This study evaluated the effect of amino acids balanced at a specific lysine ratio with exogenous protease supplementation and reduced CP levels in the broiler diet. In total, 480 straight-run broiler chicks (Ross-308) were used for the study. A completely randomized design in the factorial arrangement was applied, with 4 treatments of 6 replicates with 20 birds each. Treatments consisted of 2 lysine ratios (100% and 110%; factor 1) without or with exogenous protease supplementation (200 g/ton; factor 2). Twenty percent reduction of CP from standard requirements of Ross-308 (18.4% vs 23% in starter and 17.2% vs 21.5% in grower phase) was made in all the treatment diets. Growth performance (feed intake, weight gain, feed conversion ratio, livability), carcass traits (live and carcass weight, carcass yield, heart, liver, gizzard, leg quarter, breast weight, and abdominal fat percentage), gut morphology (villus height, crypt depth, and villus height and crypt depth ratio), and serum metabolites (total protein, albumin, and uric acid) were evaluated. A significant interaction (p ≤ 0.05) for lysine ratio and protease was noted in body weight gain, feed conversion ratio, carcass traits, and gut morphology throughout the experimental period yet a non-significant (p > 0.05) influence was observed on serum metabolites. It was concluded that the broiler diet with a net reduction of 20% CP from Ross-308 standards, balanced for amino acids at lysine ratio 110% and supplemented with exogenous protease, could be used to improve growth performance, intestinal health, and carcass traits.

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The authors declare that all the data and materials used in this study comply with field standards and are available on demand.

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Authors and Affiliations

  1. Department of Animal Nutrition, Faculty of Animal Production and Technology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan

    Muhammad Adeel Maqsood, Ehsaan Ullah Khan, Shafqat Nawaz Qaisrani, Muhammad Afzal Rashid, Azhar Nazir & Hammad Talib

  2. Department of Poultry Production, Faculty of Animal Production and Technology, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan

    Muhammad Shabir Shaheen & Sohail Ahmad

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  1. Muhammad Adeel Maqsood
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  2. Ehsaan Ullah Khan
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  8. Sohail Ahmad
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Contributions

MAM conducted this study and write the manuscript as part of his M.Phil. research work under the supervision of EUK; SNQ and SA are co-advisors. MSS, AN, and HT helped in biological trial and lab work. EUK, SNQ, and MAR help in reviewing the manuscript. SA helped in the statistical analysis and formatting of the manuscript. All authors read and approved the final manuscript for publication.

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Correspondence to Sohail Ahmad.

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All the procedures performed in this study were in accordance with the ethical standards of the University of Veterinary and Animal Sciences, Lahore, Pakistan, and approval was granted by the Animal Ethical Review Committee.

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Maqsood, M.A., Khan, E.U., Qaisrani, S.N. et al. Interactive effect of amino acids balanced at ideal lysine ratio and exogenous protease supplemented to low CP diet on growth performance, carcass traits, gut morphology, and serum metabolites in broiler chicken. Trop Anim Health Prod 54, 186 (2022). https://doi.org/10.1007/s11250-022-03184-w

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