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Influence of eCG and breed on the number of oocytes collected and the production of in vitro embryos of young goats during the reproductive season

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Abstract

The objective of this study was to determine the effect of breed and equine chorionic gonadotropin (eCG) on ovarian response and in vitro embryo production from young goats. Thirty-one (12 Alpine, 10 Nubian, and 9 Saanen) were randomly assigned into three treatments of eCG (T1, 0 IU; T2, 500 IU; and T3, 1000 IU). Alpine goats showed the highest amount and largest size of follicles (P = 0.003). The effect of eCG dose 24 h post application was significant (P < 0.05), and was superior in goats undergoing T2. The aspiration rate of cumulus–oocyte complexes (COC) was 34% (P > 0.05), except for percentage of denuded oocytes, which obtained the highest number (P = 0.003) in the Saanen goats. The same difference was found (P = 0.02) in oocytes grade III in T2 and T3, with 42.5 and 37.9% respectively. In vitro embryo production was 80.0% of IVF/cleavage in the Alpine goats (P = 0.003). Embryo production was the greatest for T2 (69.2%; P = 0.004). T3 goats had higher percentage of morula stage (66.6%; P = 0.030). It is concluded that the application of eCG has a significant effect on the ovarian status, and quality and quantity of embryos with a differential response depending on the breed.

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Acknowledgments

The authors thank CONACYT for the support of scholarship granted to the first author within the program of Masters in Animal Science Agronomy-Veterinary Graduate of the UANL, also for the support program for scientific and technological research, PAICYT-UANL-CT249-15, and for the financing of the project, and to Dr. Juan Francisco Villarreal Arredondo, for his infinite support to carry out this project. The authors are therefore very grateful to Prof. Dr. Nicolas Lopez-Villalobos for his substantial input in providing an English text version of the present manuscript.

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Authors and Affiliations

  1. Posgrado Conjunto Facultad de Agronomía-Facultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma de Nuevo León, Campus de Ciencias Agropecuarias, 66050, General Escobedo, N.L., Mexico

    Gabriela Lisset Montes-Quiroz, Fernando Sánchez-Dávila, Rogelio A. Ledezma-Torres, Rubén Cervantes-Vega, Nestor Arce-Vázquez, Estela Garza-Brenner & Hugo Bernal-Barragán

  2. Facultad de Agronomía, Unidad Académica Marín, Laboratorio de Reproducción Animal, Universidad Autónoma de Nuevo León, 66700, Marín, N.L., Mexico

    Fernando Sánchez-Dávila

  3. Unión Ganadera Regional de Nuevo León, Centro de Biotecnología Reproductiva, General Bravo, N.L., Mexico

    David Domínguez-Díaz

  4. Centro Universitario UAEM Temascaltepec, Universidad Autónoma del Estado de México, 51300, Temascaltepec, Mexico

    José Fernanco Vázquez-Armijo

  5. Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia

    Juraj Grizelj

Authors
  1. Gabriela Lisset Montes-Quiroz
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  2. Fernando Sánchez-Dávila
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  3. David Domínguez-Díaz
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  4. José Fernanco Vázquez-Armijo
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  5. Juraj Grizelj
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  6. Rogelio A. Ledezma-Torres
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  7. Rubén Cervantes-Vega
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  8. Nestor Arce-Vázquez
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  9. Estela Garza-Brenner
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  10. Hugo Bernal-Barragán
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Corresponding author

Correspondence to Fernando Sánchez-Dávila.

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This study was approved by the Bioethics and Animal Welfare Committee of the Facultad de Medicina Veterinaria y Zootecnia of the Universidad Autónoma de Nuevo León (Act. No. 18).

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The authors declare that they have no conflict of interest.

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Montes-Quiroz, G.L., Sánchez-Dávila, F., Domínguez-Díaz, D. et al. Influence of eCG and breed on the number of oocytes collected and the production of in vitro embryos of young goats during the reproductive season. Trop Anim Health Prod 51, 2521–2527 (2019). https://doi.org/10.1007/s11250-019-01972-5

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