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Determination of antibiotic resistance pattern and virulence genes in Escherichia coli isolated from bovine with subclinical mastitis in southwest of Iran

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Abstract

The aims of the present study were to investigate the prevalence of some virulence genes and also determine the antimicrobial resistance pattern of E. coli isolated from bovine with subclinical mastitis. The milk of 502 cows was collected from 8 dairy herds in the southwest of Iran. Conventional biochemical tests were used for identification of E. coli at the species level. Antimicrobial susceptibility patterns of E. coli isolates were determined by disc agar diffusion method and polymerase chain reaction (PCR) was used for detection of seven virulence genes including f17A, afaE-8, afaD-8, eaeA, cnf1, cnf2, and iucD. Seventy (13.94%) isolates of E. coli were identified in 502 milk samples. The highest rate of resistance was observed against tetracycline (18.6%), while none of the isolates were resistant to streptomycin. Eight (11.5%) out of 70 E. coli isolates carried at least one of the virulence genes. The afaD-8 was the most prevalent gene detected in 5 (7.1%) isolates. The afaE-8, iucD, and eaeA were detected in 3, 3, and 2 isolates respectively. Low prevalence of virulence factors may be indicating that most of the E. coli isolates originated from the commensal flora of cows and enter to the udders via environment contamination with feces.

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Funding

This study has been supported by Deputy of Research and Technology, Yasuj University of Medical Sciences.

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Authors and Affiliations

  1. Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran

    Masoud Marashifard & Seyed Ali Asghar Malek Hosseini

  2. Department of Basic Sciences, Yasooj Branch, Islamic Azad University, Yasooj, Iran

    Zahra Karimi Aliabad

  3. Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

    Davood Darban-Sarokhalil

  4. Faculty of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran

    Mehdi Mirzaii

  5. Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

    Seyed Sajjad Khoramrooz

Authors
  1. Masoud Marashifard
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  2. Zahra Karimi Aliabad
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  3. Seyed Ali Asghar Malek Hosseini
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  4. Davood Darban-Sarokhalil
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  5. Mehdi Mirzaii
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  6. Seyed Sajjad Khoramrooz
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Corresponding author

Correspondence to Seyed Sajjad Khoramrooz.

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This study was approved by the ethical committee of Yasuj University of Medical Sciences (ethical code number: IR.YUMS.REC.1395.132).

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The authors declare that they have no conflict of interest.

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Marashifard, M., Karimi Aliabad, Z., Malek Hosseini, S.A.A. et al. Determination of antibiotic resistance pattern and virulence genes in Escherichia coli isolated from bovine with subclinical mastitis in southwest of Iran. Trop Anim Health Prod 51, 575–580 (2019). https://doi.org/10.1007/s11250-018-1726-0

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  • DOI: https://doi.org/10.1007/s11250-018-1726-0

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