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Synchronization of ovulation with human chorionic gonadotropin in lactating dairy cows with ovarian cysts during heat stress

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Abstract

A study was conducted during hot season to determine the effect of synchronization of ovulation with human chorionic gonadotropin (hCG) on fertility of lactating dairy cows with ovarian cysts. Non cyclic Holstein dairy cows (n = 80) were stratified by parity and diagnosed as having an ovarian cyst. The cows were further identified as follicular or luteal cysts according to the plasma progesterone (P4) concentration and the cystic image of ultrasonography. Cystic cows were randomly assigned to receive treatments (Ovsynch as the control or Ovsynch plus 3000 IU hCG). All cows were artificially inseminated at 16–18 h after the second gonadotropin releasing hormone injection. Cows supplemented with hCG had a greater number of corpus luteum (1.8 ± 0.2 and 0.8 ± 0.3; P < 0.05) and had greater P4 concentration on day 12 than those control cows (6.3 ± 0.3 and 3.9 ± 0.4 ng/ml; P < 0.05). Concentration of cortisol did not differ between groups of cystic cows. No significant differences were found in overall conception rates between the treatments; however, significantly greater conception rate (P = 0.03) was observed in cows with luteal cysts receiving Ovsynch plus hCG. This study highlights that administration of hCG following the Ovsynch-based timed artificial insemination (AI) is more effective than the control Ovsynch by which the hCG affects corpus luteum (CL) development, P4 concentration, and thus improves conception rate in dairy cows with luteal cysts.

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References

  • Alnimer, M.A., Alfataftah, A.A., and Ababneh, M.M., 2011. A comparison of fertility with a cosynch protocol versus a modified Ovsynch protocol which included estradiol in lactating dairy cows during the summer season in Jordan. Animal Reproduction Science, 8, 32–39.

    Google Scholar 

  • Ambrose, J.D., Drost, M., Monson, R.L., Rutledge, J.J., Leibfried-Rutledge, M.L., Thatcher, M.J., Kassa, T., Binelli, M., Hansen, P.J., Chenoweth, P.J., and Thatcher, W.W., 1999. Efficacy of timed embryo transfer with fresh and frozen in vitro produced embryos to increase pregnancy rates in heat-stressed dairy cattle. Journal of Dairy Science, 82, 2369–2376.

    Article  CAS  PubMed  Google Scholar 

  • Armstrong, D.V., 1994. Heat stress interactions with shade and cooling. Journal of Dairy Science 77, 2044–2050.

    Article  CAS  PubMed  Google Scholar 

  • Bartolome, J.A., Archbald, L.F., Morresey, P., Hernandez, J., Tran, T., Kelbert, D., Long, K., Risco, C.A., and Thatcher, W.W., 2000. Comparison of synchronization of ovulation and induction of estrus as therapeutic strategies for bovine ovarian cysts in the dairy cow. Theriogenology, 53, 815–825.

    Article  CAS  PubMed  Google Scholar 

  • Bartolome, J., Melendez, P., Kelbert, D., Swift, K., McHale, J., Hernandez, J., Silvestre, F., Risco, C., Arteche, A., Thatcher, W., Archbald, L., 2005. Strategic use of gonadotrophin-releasing hormone (GnRH) to increase pregnancy rate and reduce pregnancy loss in lactating dairy cows subjected to synchronization of ovulation and timed insemination. Theriogenology, 63, 1026–1037.

    Article  CAS  PubMed  Google Scholar 

  • Crane, M.B., Bartolome, J., Melendez, P., deVries, A., Risco, C., Archbald, L.F., 2006. Comparison of synchronization of ovulation with timed insemination and exogenous P4 as therapeutic strategies for ovarian cysts in lactating dairy cows. Theriogenology, 65, 1563–1574.

    Article  CAS  PubMed  Google Scholar 

  • De la Sota, R.L., Burke, J.M., Risco, C.A., Moreira, F., DeLorenzo, M.A., and Thatcher, W.W., 1998. Evaluation of timed insemination during summer heat stress in lactating dairy cattle. Theriogenology, 49, 761–770.

    Article  PubMed  Google Scholar 

  • De Rensis, F., Gorrieri, F., Bottarelli, E., Lopez-Gatius, F., and Valentini, R., 2008. Inducing ovulation with hCG improves the fertility of dairy cows during the warm season. Theriogenology, 69, 1077–1082.

    Article  PubMed  Google Scholar 

  • De Rensis, F., Lopez-Gatius, F., Garcia-ispierto, I., Techakumpu, M., 2010. Clinical use of human chorionic gonadotropin in dairy cows: an update. Theriogenology, 73, 1001–1008.

    Article  PubMed  Google Scholar 

  • De Rensis, F., Scaramuzzi, R.J., 2003. Heat stress and seasonal effects on reproduction in the dairy cows-a review. Theriogenology, 60, 1139–1151.

    Article  PubMed  Google Scholar 

  • Fricke, P.M., Reynolds, L.P., and Redmer, D.A., 1993. Effect of human chorionic gonadotropin administered early in the estrous cycle on ovulation and subsequent luteal function in cows. Journal of Animal Science 71, 1242–1246.

    CAS  PubMed  Google Scholar 

  • Friedman, E., Roth, Z., Voet, H., Lavon, Y., Wolfenson, D., 2012. Progesterone supplementation postinsemination improves fertility of cooled dairy cows during the summer. Journal of Dairy Science 95, 3092–3099.

    Article  CAS  PubMed  Google Scholar 

  • Garverick, H.A., 1997. Ovarian follicular cysts in dairy cows. Journal of Dairy Science, 80, 995–1004.

    Article  CAS  PubMed  Google Scholar 

  • Gendelman, M., Aroyo, A., Yavin, S., Roth, Z., 2010. Seasonal effects on gene expression, cleavage timing, and developmental competence of bovine preimplantation embryos. Reproduction, 140, 73–82.

    Article  CAS  PubMed  Google Scholar 

  • Gundling, N., Drews, S., Hoedemaker, M., 2009. Comparison of two different programmes of ovulation synchronization in the treatment of ovarian cysts in dairy cows. Reproduction in Domestic Animals, doi: 10.1111/j.1439-0531.2009.01342.x.

    PubMed  Google Scholar 

  • Hamilton, S.A., Garverick, H.A., Keisler, D.H., Xu, Z.Z., Loos, K., Youngquist, R.S., Salfen, B.E., 1995. Characterization of ovarian follicular cysts and associated endocrine profiles in dairy cows. Biology of Reproduction, 53, 890–898.

    Article  CAS  PubMed  Google Scholar 

  • Hanlon, D.W., Jarratt, G.M., Davidson, P.J., Millar, A.J., Douglas, V.L., 2005. The effect of hCG administration five days after insemination on the first service conception rate of anestrous dairy cows. Theriogenology, 63, 1938–1945.

    Article  CAS  PubMed  Google Scholar 

  • Hansen, P.J., 2007. Exploitation of genetic and physiological determinants of embryonic resistance to elevated temperature to improve embryonic survival in dairy cattle during heat stress. Theriogenology, 68, 242–249.

    Article  Google Scholar 

  • Hansen, P.J., 2013. Cellular and molecular basis of therapies to ameliorate effects of heat stress on embryonic development in cattle. Animal Reproduction, 10, 322–333.

    Google Scholar 

  • Hatler, T.B., Hayes, S.H., Laranja da Fonseca, L.F., Silvia, W.J., 2003. Relationship between endogenous progesterone and follicular dynamics in lactating dairy cows with ovarian follicular cysts. Biology of Reproduction, 69, 218–223.

    Article  CAS  PubMed  Google Scholar 

  • Howell, J.L., Fuquay, J.W., Smith, A.E., 1994. Corpus luteum growth and function in lactating Holstein cows during spring and summer. Journal of Dairy Science, 77, 735–739.

    Article  CAS  PubMed  Google Scholar 

  • Jordan, E.R., 2003. Effects of heat stress on reproduction. Journal of Dairy Science, 86 (E. Suppl.), E104–E114.

  • Kawate, N., 2004. Studies on the regulation of expression of luteinizing hormone receptor in the ovary and the mechanism of follicular cyst formation in ruminants. Journal of Reproduction and Development, 57, 267–272.

    Article  Google Scholar 

  • Kawate, N., Watanabe, K., Uenaka, K., Takahashi, M., Inaba, T., Tamada, H., 2011. Comparison of plasma concentrations of estradiol-17β and progesterone, and conception in dairy cows with cystic ovarian progesterone diseases between Ovsynch and Ovsynch plus CIDR timed AI protocols. Journal of Reproduction and Development, 57, 267–272.

    Article  CAS  PubMed  Google Scholar 

  • Keskin, A., Yilmazbas-Mecitoglu, G., Gumen, A., Karakaya, E., Darici, R., Okut, H., 2010. Effect of hCG vs. GnRH at the beginning of the Ovsynch on first ovulation and conception rates in cyclic lactating dairy cows. Theriogenology, 74, 602–607.

    Article  CAS  PubMed  Google Scholar 

  • Kim, I.H., Suh, G.H., Kim, U.H., Kang, H.G., 2006. A CIDR-based timed AI protocol can be effectively used for dairy cows with follicular cysts. Animal Reproduction Science, 95, 206–213.

    Article  CAS  PubMed  Google Scholar 

  • Kodokawa, H., Sakatani, M., Hansen, P.J., 2012. Perspectives on improvement of reproduction in cattle during heat stress in a future Japan. Animal Science Journal, 83, 439–445.

    Article  Google Scholar 

  • Lopez-Gatius, F., 2003. Is fertility declining in dairy cattle?: a retrospective study in northeastern Spain. Theriogenology, 60, 89–99.

    Article  CAS  PubMed  Google Scholar 

  • Lopez-Gatius, F., Lopez-Bejar, M., 2002. Reproductive performance of dairy cows with ovarian cysts after different GnRH and cloprostenol treatments. Theriogenology, 58, 1337–1348.

    Article  CAS  PubMed  Google Scholar 

  • Moonmanee, T., Navanukraw, C., Uriyapongson, S., Kraisoon, A., Aiumlamai, S., Guntaprom, S., Rittirod, T., Borowicz, P.P., Redmer, D.A., 2013. Relationships among vasculature, mitotic activity, and endothelial nitric oxide synthase (eNOS) in bovine antral follicles of the first follicular wave. Domestic Animal Endocrinology, 45, 11–21.

    Article  CAS  PubMed  Google Scholar 

  • Navanukraw, C., Boonsom, A., Guntaprom, S., Uriyapongson, S., Wachirapakorn, C., 2009. Plant oil supplementation in dietary concentrate improves milk yield, ovarian function and uterine health of postpartum dairy cows in a tropical environment. Journal of Dairy Science, 92, (E-Suppl. 1), 72

    Google Scholar 

  • Navanukraw, C., Redmer, D.A., Reynolds, L.P., Kirsch, J.D., Grazul-Bilska, A.T., Fricke, P.M., 2004. A modified presynchronization protocol improves fertility to timed artificial insemination in lactating dairy cows. Journal of Dairy Science, 87, 1551–1557.

    Article  CAS  PubMed  Google Scholar 

  • NRC, 2001. Nutrient Requirements of Dairy Cattle, 7th ed. National Academy Press, Washington, D.C.

  • Payton, R.R., Romar, R., Coy, P., Saxton, A.M., Lawrence, J.L., Edwards, J.L., 2004. Susceptibility of bovine germinal vesicle-stage oocytes from antral follicles to direct effects of heat stress in vitro. Biology of Reproduction. 71:1303–1308.

    Article  CAS  PubMed  Google Scholar 

  • Peter, A.T., 2004. An update on cystic ovarian degeneration in cattle. Reproduction in Domestic Animal 39, 1–7.

    Article  CAS  Google Scholar 

  • Probo, M., Comin, A., Mollo, A., Cairoli, F., Stradaioli, G., Veronesi, M.C., 2011. Reproductive performance of dairy cows with luteal or follicular ovarian cysts after treatment with buserelin. Animal Reproduction Science, 127, 135–139.

    Article  CAS  PubMed  Google Scholar 

  • Roth, Z., 2014. Effect of heat stress on ovarian functions and embryonic development: mechanism and potential strategies to alleviate these effects in dairy cows. In: J.L. Juengel, A. Miyamoto, C.Price, L.P. Reynolds, M.F. Smith, and R. Webb (eds), Proceedings of the ninth international symposium on reproduction in domestic ruminants, Obihiro, Hokkaido, Japan, 2014, (Society for Reproduction and Fertility, England), 193–208.

  • Santos, J.E., Thatcher, W.W., Pool, L., Overton, M.W., 2001. Effect of human chorionic gonadotropin on luteal function and reproductive performance of high-producing lactating Holstein dairy cows. Journal of Animal Science, 79, 2188–2194.

    Google Scholar 

  • Sartori, R., Sartor-Bergfelt, R., Mertens S.A., Guenther, J.N., Parrish, J.J., Wiltbank, M.C., 2002. Fertilization and early embryonic development in heifers and lactating cows in summer and lactating and dry cows in winter. Journal of Dairy Science, 85, 2803–2812.

    Article  CAS  PubMed  Google Scholar 

  • Souza, A.H., Ayres, H., Ferreira, R.M., Wiltbank, M.C., 2008. A new presynchronization system (Double-Ovsynch) increases fertility at first postpartum timed AI in lactating dairy cows. Theriogenolgy, 70, 208–215.

    Article  CAS  Google Scholar 

  • Steel, R.G.D., Torrie, J.H., Dickey D. 1997. Principles and procedures of statistics: a biometrical approach. 3rd Ed. New York: McGraw-Hill.

    Google Scholar 

  • Thatcher, W.W., Bilby, T.R., Bartolome, J.A., Silvestre, F., Staples, C.R., Santos, J.E.P., 2006. Strategies for improving fertility in the modern dairy cow. Theriogenology, 65, 30–44.

    Article  CAS  PubMed  Google Scholar 

  • Todoroki, J., Kaneko, H., 2006. Formation of follicular cysts in cattle and therapeutic effects of controlled internal drug release. Journal of Reproduction Development, 52, 1–11.

    Article  CAS  PubMed  Google Scholar 

  • Ullah, G., Fuquay, J.W., Keawhoong, T., Clark, B.L., Pogue, D.E., Murphy, E.J., 1996. Effect of gonadotrophin-releasing hormone at estrus on subsequent luteal function and fertility in lactating Holstein during heat stress. Journal of Dairy Science, 79, 1950–1953.

    Article  CAS  PubMed  Google Scholar 

  • Vanholder, T., Opsomer, G., de Kruif, A., 2006. Aetiology and pathogenesis of cystic ovarian follicles in dairy cattle: a review. Reproduction Nutrition Development, 46, 105–119.

    Article  CAS  Google Scholar 

  • Willard, S., Gandy, S., Bowers, S., Graves, K., Elias, A., Whisnant, C., 2003. The effect of GnRH administration post-insemination on serum concentrations of progesterone and pregnancy rates in dairy cattle exposed to mild summer heat stress. Theriogenology, 59, 1799–1810.

    Article  CAS  PubMed  Google Scholar 

  • Wiltbank, M.C., Gumen, A., Sartori, R., 2002. Physiological classification of anovulatory conditions in cattle. Theriogenology, 57, 21–52.

    Article  CAS  PubMed  Google Scholar 

  • Wise, M.E., Armstrong, D.V., Huber, J.T., Hunter, R., Wiersma, F., 1988. Hormonal alterations in the lactating dairy cow in response to thermal stress. Journal of Dairy Science, 71, 2480–2485.

    Article  CAS  PubMed  Google Scholar 

  • Wolfenson, D., Lew, B.J., Thatcher, W.W., Graber, Y., Medan, R., 1997. Seasonal and acute heat stress effects on steroid production by dominant follicles in cows. Animal Reproduction Science, 47, 9–19.

    Article  CAS  PubMed  Google Scholar 

  • Wolfenson, D., Roth, Z., Meidan, R., 2000. Impaired reduction in heat-stress cattle: basic and applied aspects. Animal Reproduction Science, 60, 535–547.

    Article  PubMed  Google Scholar 

  • Zeron, Y., Ocheretny, A., Kedar, O., Borochov, A., Sklan, D., Arav, A., 2001. Seasonal changes in bovine fertility: relation to developmental competence of oocytes, membrane properties and fatty acid composition of follicles. Reproduction, 121, 447–454.

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This study was financially supported by Thailand Research Fund (TRF), National Research Council Thailand (NRCT), National Research University (NRU) and Increased Production Efficiency and Meat Quality of Native Beef and Buffalo Research Group, Khon Kaen University, Thailand.

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We have no conflict of interest to declare.

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Authors and Affiliations

  1. Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand

    Chainarong Navanukraw, Vilaivan Khanthusaeng & Aree Kraisoon

  2. Agriculture Biotechnology Research Center for Sustainable Economy (ABRCSE), Khon Kaen University, Khon Kaen, 40002, Thailand

    Chainarong Navanukraw

  3. Department of Agricultural Extension, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand

    Duangkamon Suwannarit

  4. Department of Surgery and Theriogenology, Faculty of Veterinary Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand

    Chaiwat Jarassaeng & Suneerat Aiumlamai

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  1. Chainarong Navanukraw
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  2. Vilaivan Khanthusaeng
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  3. Aree Kraisoon
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Correspondence to Chainarong Navanukraw.

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Navanukraw, C., Khanthusaeng, V., Kraisoon, A. et al. Synchronization of ovulation with human chorionic gonadotropin in lactating dairy cows with ovarian cysts during heat stress. Trop Anim Health Prod 47, 945–951 (2015). https://doi.org/10.1007/s11250-015-0813-8

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  • DOI: https://doi.org/10.1007/s11250-015-0813-8

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