Abstract
A study was carried out to establish the prevalence of subclinical mastitis (SCM) in smallholder dairy farms in Ismailia, Egypt. A total of 340 milking cows and buffaloes were sampled from 60 farms, and 50 nasal swabs were collected from consenting farm workers. Milk samples were subjected to California mastitis test (CMT) and the positive samples were examined by bacterial culture and PCR to identify etiological agents. Based on CMT, the prevalence of SCM was 71.6 % in cattle and 43.5 % in buffaloes while the prevalence was 25.2 % at cow-quarter level and 21.7 % at buffaloes-quarter level. Bacteriological analysis showed that the most frequently identified bacteria were Staphylococcus (S.) aureus (38.3 %) and Streptococcus (Str.) agalactiae (20 %). The diagnostic sensitivity of PCR compared to bacterial culture was superior with S. aureus and Str. agalactiae detection being 41 and 22.6 %, respectively. Furthermore, methicillin-resistant S. aureus (MRSA) strains occurred in 52.2 and 45 % of isolates of animals and workers, respectively. Subclinical mastitis due to S. aureus and Str. agalactiae is endemic in smallholder dairy herds in Ismailia. The occurrence of MRSA in animals and workers highlights a need for wide epidemiological studies of MRSA and adopting control strategies.
Similar content being viewed by others
References
Ahmadi, M., Razavi Rohani, S. M. and Ayremlou, N., 2009. Evaluation of Streptococcus agalactiae detection by PCR in Milk and its comparison to other microbiological methods. Iranian journal of microbiology, 1(4), 28–31.
Aidaros, H., 2005. Global perspectives - the Middle East: Egypt. Revue scientifique et technique (International Office of Epizootics), 24 (2), 589–596.
Amin, A. S., Hamouda, R. H. and Abdel-All, A. A., 2011. PCR Assays for Detecting Major Pathogens of Mastitis in Milk Samples. World Journal of Dairy & Food Sciences, 6 (2), 199–206.
Balaban, N. and Rasooly, A., 2000. Staphylococcal enterotoxins. International Journal of Food Microbiology, 61 (1), 1–10.
Barkema, H.W., Schukken, Y.H. and Zadoks, R.N., 2006. Invited review: the role of cow, pathogen, and treatment regimen in the therapeutic success of bovine Staphylococcus aureus mastitis. Journal of Dairy Science, 89, 1877–1895.
Benić, M., Habrun, B. and Kompes, G., 2012. Clinical and Epidemiological Aspects of Cow Mastitis Caused by Staphylococcus aureus and its Methicillin-Resistant Strains. Rad Hrvatske akademije znanosti i umjetnosti. Medicinske znanosti, 37, 113–121.
Daka, D., Solomon, G/ silassie and Yihdego, D., 2012. Antibiotic-resistance Staphylococcus aureus isolated from cow’s milk in the Hawassa area, South Ethiopia. Annals of Clinical Microbiology and Antimicrobials, 11, 26–37.
Elias, A. O., Cortez, A., Brandão, P. E., da Silva, R. C. and Langoni, H., 2012. Molecular detection of Streptococcus agalactiae in bovine raw milk samples obtained directly from bulk tanks. Research in Veterinary Science, 93(1), 34–38.
Falagas, M. E., Karageorgopoulos, D. E., Leptidis, J. and Korbila, I. P., 2013. MRSA in Africa: Filling the Global Map of Antimicrobial Resistance. PloS one, 8 (7), e68024.
Fox, L. K. and Gay, J. M., 1993. Contagious mastitis. Veterinary Clinics of North America: Food Animal Practice, 9, 475–487.
Getahun, K., Kelay, B., Bekana, M. and Lobago, F., 2008. Bovine mastitis and antibiotic resistance patterns in Selalle smallholder dairy farms, central Ethiopia. Tropical Animal Health and Production, 40, 261–8.
Gianneechini, R., Concha, C., Rivero, R., Delucci, I. and Moreno López, J., 2002. Occurrence of clinical and sub-clinical mastitis in dairy herds in the West Littoral Region in Uruguay. Acta Veterinaria Scandinavica, 43, 221–230.
Joshi, S. and Gokhale, S., 2006. Status of mastitis as an emerging disease in improved and periurban dairy farms in India. Annals of the New York Academy of Sciences, 1081 (1), 74–83.
Juhász-Kaszanyitzky, É., Jánosi, S., Somogyi, P., Dán, Á., vanderGraaf van Bloois, L., van Duijkeren, E. and Wagenaar, J. A., 2007. MRSA transmission between cows and humans. Emerging Infectious Diseases, 13 (4), 630–632.
Keane, O. M., Budd, K. E., Flynn, J. and McCoy, F., 2013. Increased detection of mastitis pathogens by real-time PCR compared to bacterial culture. Veterinary Record, 173(11), 268–268.
Koskinen, M.T., Wellenberg, G.J., Sampimon, O.C., Holopainen, J., Rothkamp, A., Salmikivi, L., Van Haeringen, W.A., Lam, T.J.G.M. and Pyörälä, S., 2010. Field comparison of real-time polymerase chain reaction and bacterial culture for identification of bovine mastitis bacteria. Journal of Dairy Science, 93, 5707–5715.
Lee, J. H. 2003. Methicillin (oxacillin)-resistant Staphylococcus aureus strains isolated from major food animals and their potential transmission to humans. Applied and Environmental Microbiology, 69:6489–6494.
Lee, K. H., Lee, J. W., Wang, S. W., Liu, L. Y., Lee, M. F., Chuang, S. T., Shy, Y.M., Chang, C.L., Wu, M.C. and Chi, C. H., 2008. Development of a novel biochip for rapid multiplex detection of seven mastitis-causing pathogens in bovine milk samples. Journal of veterinary diagnostic investigation, 20(4), 463–471.
Mahmmod, Y. S., Toft, N., Katholm, J., Grønbæk, C. and Klaas, I. C., 2013. Bayesian estimation of test characteristics of real-time PCR, bacteriological culture and California mastitis test for diagnosis of intramammary infections with Staphylococcus aureus in dairy cattle at routine milk recordings. Preventive Veterinary Medicine, 112 (3), 309–317.
Makovec, J.A. and Ruegg, P.L., 2003. Results of milk samples submitted for microbiological examination in Wisconsin from 1994 to 2001. Journal of Dairy Science, 86:3466–3472.
Mdegela, R. H., Ryoba, R., Karimuribo, E. D., Phiri, E. J., Løken, T., Reksen, O., Mtengeti, E. and Urio, N. A., 2009. Prevalence of clinical and subclinical mastitis and quality of milk in smallholder dairy farms in Tanzania. Journal of the South African Veterinary Association, 80 (3), 163–168.
Meiri-Bendek, I., Lipkin, E., Friedmann, A., Leitner, G., Saran, A., Friedman, S. and Kashi, Y., 2002. A PCR-Based Method for the Detection of Streptococcus agalactiae in Milk. Journal of Dairy Science, 85 (7), 1717–1723.
Monday, S.R. and Bohach, G.A., 1999. Use of multiplex PCR to detect classical and newly described pyrogenic toxin genes in staphylococcal isolates. Journal of Clinical Microbiology, 37, 3411–3414.
Moroni, P., Sgoifo Rossi, C., Pisoni, G., Bronzo, V., Castiglioni, B. and Boettcher, P. J., 2006. Relationships between somatic cell count and intramammary infection in buffaloes. Journal of Dairy Science, 89 (3), 998–1003.
Murakami, K., Minamide, W., Wada, K., Nakamura, E., Teraoka, H. and Watanabe, S., 1991. Identification of methicillin-resistant strains of staphylococci by polymerase chain reaction. Journal of Clinical Microbiology, 29:2240–2244.
National Mastitis Council, 1999. Laboratory and Field Handbook on Bovine Mastitis. 2nd Edition. National Mastitis Council, Madison, Wisconsin, USA.
Nielen, M., Schukken, Y. H., Van De Broek, J., Brand, A., Deluyker, H. A. and Maatje, K., 1993. Relations between on-line electrical conductivity and daily milk production on a low somatic cell count farm. Journal of Dairy Science, 76, 2589–2596.
Paterson, G. K., Harrison, E. M., Craven, E. F., Petersen, A., Larsen, A. R., Ellington, M. J., Török, M. E., Peacock, S. J., Parkhill, J., Zadoks, R. N. and Holmes, M. A., 2013. Incidence and Characterisation of Methicillin-Resistant Staphylococcus aureus (MRSA) from Nasal Colonisation in Participants Attending a Cattle Veterinary Conference in the UK. PloS one. 8 (7), e68463.
Pexara, A., Solomakos, N. and Govaris, A., 2013. Prevalence of methicillin-resistant Staphylococcus aureus in milk and dairy products. Journal of the Hellenic Veterinary Medical Society, 64 (1), 17–34.
Phuektes, P., Mansell, P.D. and Browning, G.F., 2001. Multiple polymerase chain reaction assay for simultaneous detection of Staphylococcus aureus and streptococcal causes of bovine mastitis. Journal of Dairy Science, 84,1140–1148.
Pinto, T.C.A., Costa, N.S., Souza, A.R.V., Silva, L.G.D., Corrêa, A.B..D.A., Fernandes, F.G., Oliveira I.C., Mattos M.C., Rosado A.S. and Benchetrit, L.C., 2013. Distribution of serotypes and evaluation of antimicrobial susceptibility among human and bovine Streptococcus agalactiae strains isolated in Brazil between 1980 and 2006. Brazilian Journal of Infectious Diseases, 17(2), 131–136.
Riffon, R., Sayasith, K., Lhalil, H., Dubreuil, P., Drolet, M. and Lagace, J., 2001. Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR. Journal of Clinical Microbiology, 39, 2584–2589.
Soo Ko, K., Peck, K. R., Sup Oh, W., Lee, N. Y., Hiramatsu, K. and Song. J. H., 2005. Genetic differentiation of methicillin-resistant Staphylococcus aureus strains from Korea and Japan. Microbial Drug Resistance,11 (3), 279–286.
Taponen, S., Salmikivi, L., Simojoki, H., Koskinen, M. T. and Pyörälä, S., 2009. Real-time polymerase chain reaction-based identification of bacteria in milk samples from bovine clinical mastitis with no growth in conventional culturing. Journal of dairy science, 92(6), 2610–2617.
Tsonev, P., Kamburov, G. and G’l’binov, G., 1975. Interbreed and interspecies variation in the occurrence of subclinical mastitis in cows and buffaloes. Veterinarno-meditsinski nauki, 12 (9), 37–40.
Viera, A. J. and Garrett, J. M., 2005. Understanding interobserver agreement: the kappa statistic. Family Medicine, 37(5), 360–363.
Zadoks, R. N., Middleton, J. R., McDougall, S., Katholm, J. and Schukken, Y. H., 2011. Molecular epidemiology of mastitis pathogens of dairy cattle and comparative relevance to humans. Journal of Mammary Gland Biology and Neoplasia, 16(4), 357–372.
Acknowledgments
We are grateful to Dr. Mohammad Nagi Elsheery, head of the Department of Animal Medicine, for his support and constructive advice. We wish to thank Dr. Armanda D. S. Bastos, Department of Zoology and Entomology, University of Pretoria, South Africa, for her advice on the manuscript.
Conflict of interest
None.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Elhaig, M.M., Selim, A. Molecular and bacteriological investigation of subclinical mastitis caused by Staphylococcus aureus and Streptococcus agalactiae in domestic bovids from Ismailia, Egypt. Trop Anim Health Prod 47, 271–276 (2015). https://doi.org/10.1007/s11250-014-0715-1
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11250-014-0715-1