Abstract
Bauhinia holophylla is a woody plant found in the Brazilian Cerrado used in folk medicine to treat diabetes. Lectins are glycoproteins with several biotechnological applications and play important roles in plant defenses and in medicine. Lectins have been produced in vitro using plant tissue culture techniques. This study shows the production and characterization of lectin from B. holophylla by callus culture. Calli were obtained from leaf segments placed on woody plant and Murashige and Skoog media supplemented with 2,4-dichlorophenoxyacetic acid (0, 4.52, 9.05, 18.10 µM) or 6-benzylaminopurine (0, 4.44, 8.88, 17.75 µM), in the presence or absence of light. The highest concentrations of lectin expressed by hemagglutination activity were observed in green and compact callus induced in woody plant medium supplemented with 6-benzylaminopurine in the presence of light, which showed specificity by all types of erythrocytes tested. The highest concentrations of lectin (128 HU mg−1) and fresh weight of callus were observed in the stationary phase (41st–60th day). Lectin was not detected in subcultured calli. d-Galactose promoted the highest inhibitory effect on the lectin activity in human erythrocytes ABO system, especially B-type. Lectin has been shown to be a stable protein, maintaining its hemagglutination activity after incubation at a wide range of temperatures, pH values and ethylenediaminetetraacetic acid concentrations.
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Acknowledgements
We thank Dr. Andreia Fonseca Silva for the botanical identification of this plant and Dávia Guimarães Pompeu and the staff member Donizetti Joel Vilas Boas for help in hemagglutination test and assistance in the field, respectively. The authors also wish to thank to Foundation for Research Support of Minas Gerais (FAPEMIG) (CRA APQ 01347-14) and National Council for Scientific and Technological Development (CNPq) (Process 444907/2014-9) for financial support.
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Communicated by Sergio J. Ochatt.
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Castro, A.H.F., da Silva Tavares, H., Pereira, S.R.F. et al. Production and characterization of lectin from Bauhinia holophylla (Fabaceae:Cercideae) calli. Plant Cell Tiss Organ Cult 134, 423–432 (2018). https://doi.org/10.1007/s11240-018-1432-7
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DOI: https://doi.org/10.1007/s11240-018-1432-7