Skip to main content
SpringerLink
Log in

Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture

  • Original Article
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

An Erratum to this article was published on 09 February 2017

Abstract

A total of 200 doubled haploids (DHs) were generated from an elite rice hybrid, ‘BS6444G’ for which an androgenic method was developed by manipulating the physical and chemical factors. The spike pretreated at 10 °C for 7–8 days was effective for callusing and green plant regeneration. The maximum callus frequency was achieved when the anthers cultured in N6 medium supplemented with 2.0 mg L−1 2,4-diclorophenoxyacetic acid, 0.5 mg L−1 6-benzylaminopurine and 3% maltose. Calli induced in N6 media also showed significant green shoot regeneration in MS medium supplemented with 0.5 mg L−1 1-napthalene acetic acid, 0.5 mg L−1 kinetin, 1.5 mg L−1 benzylaminopurine and 3% sucrose producing 210 green plants. Assessment of the ploidy status showed 95.71% fertile diploids and 4.2% polyploids; no haploids were observed. A total of 38 sequence-tagged microsatellite (STMS) markers proved able to discriminate a heterozygote from all the 200 DHs. The DHs grown in the field showed significant variation for their agronomic traits. Comparison of traits with control indicates homogeneity within each DH line and significant variance of traits between DH lines. Nine DH lines produce higher grain yield than the hybrid parent which suggests the possibility of exploiting hybrid vigor in indica rice through the development of DH lines of high yielding hybrids.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Afza R, Shen M, Zapata-Arias FJ, Xie J, Fundi HK, Lee KS, Bobadilla-Mucino E, Kodym A (2000) Effect of spikelet position on rice anther culture efficiency. Plant Sci 153:155–159

    Article  CAS  PubMed  Google Scholar 

  • Asaduzzaman M, Bari MA, Rahman MH, Khatun N, Islam MA, Rahman M (2003) In vitro plant regeneration through anther culture of five rice varieties. J Biol Sci 3:167–171

    Article  Google Scholar 

  • Bagheri N, Jelodar NB (2008) Combining ability and heritability of callus induction and green plant regeneration through anther culture. Biotechnology 7:287–292

    Article  CAS  Google Scholar 

  • Bishnoi U, Jain RK, Rohilla JS, Chowdhury VK, Gupta KR, Chowdhury JB (2000) Anther culture of recalcitrant indica × basmati rice hybrids. Euphytica 114:93–101

    Article  CAS  Google Scholar 

  • Bohorova NE, Luna B, Brito RM, Huerta LD, Hoisington DA (1995) Regeneration potential of tropical, sub tropical, mid altitude and highland maize inbreds. Maydica 40:275–281

    Google Scholar 

  • Chu CC, Wang CS, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY (1975) Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sinica 18:659–668

    Google Scholar 

  • Dewi IS, Purwoko BS, Aswidinnoor H, Somantri IH, Chozin MA (2009) Plant regeneration from anther cultures of several genotypes of indica rice tolerant to aluminum toxicity. Indones J Agric Sci 2:1–5

    Google Scholar 

  • Echavarri B, Cistue L (2016) Enhancement in androgenesis efficiency in barley (Hordeum vulgare L.) and bread wheat (Triticum aestivum L.) by the addition of dimethyl sulfoxide to the mannitol pretreatment medium. Plant Cell Tissue Organ Cult 125:11–22

    Article  CAS  Google Scholar 

  • Forster BP, Thomas WTB (2005) Doubled haploids in genetics and plant breeding. Plant Breed Rev 25:57–88

    CAS  Google Scholar 

  • Genovesi AD, Magill CW (1979) Improved rate of callus and green plant production from rice anther culture following cold shock. Crop Sci 19:662–664

    Article  Google Scholar 

  • Grewal D, Manito CH, Bartolome V (2011) Doubled haploids generated through anther culture from crosses of elite indica and japonica cultivars and/or lines of rice: large scale production, agronomic performance and molecular characterization. Crop Sci 51:2544–2553

    Article  Google Scholar 

  • Han H, Huang B (1987) Application of pollen-derived plants to crop improvement. Int Rev Cytol 107:293–313

    Article  Google Scholar 

  • Heberle-Bors E, Stöger E, Touraev A, Zarsky V, Vicente O (1996) In vitro pollen cultures: progress and perspectives. In: Mohapatra SS, Knox RB (eds) Pollen biotechnology. Gene expression and allergen characterization, Chapman and Hall, New York, pp 85–109

    Google Scholar 

  • Javed MA, Ishi T, Kamijima O, Misoo S (2007) The role of alternating culture temperatures and maltose in enhancing anther culture efficiency of salt tolerant indica rice (Oryza sativa L.) cultivars, Pokkali and Nona Bokra. Plant Biol 24:283–287

    CAS  Google Scholar 

  • Khatun R, Shahinul Islam SM, Ara I, Tuteja T, Bari MA (2012) Effect of cold treatment and different media in improving anther culture response in rice (O. sativa L) in Bangladesh. Indian J Biotechnol 11:458–463

    CAS  Google Scholar 

  • Lee YT, Lim MS, Kim, HS, Shin HT, Kim CH, Bae SH, Cho CI (1989) An anther derived new high quality variety with disease and insect resistance ‘Hwacheong byeo’. Res Rep Rural Dev Admin Rice 31(2):27–34

    Google Scholar 

  • Lenka N, Reddy GM (1994) Role of media, plant growth regulators in callusing and plant regeneration from anthers of indica rice. Proc Indian Natl Acad Sci 60: 87–92

    CAS  Google Scholar 

  • Lentini Z, Reyes P, Martinez CP, Roca WM (1995) Androgenesis of highly recalcitrant rice genotypes with maltose and silver nitrate. Plant Sci 110:127–138

    Article  CAS  Google Scholar 

  • Marassi MA, Scocchi A, Gonzalez AM (2006) Plant regeneration from rice anthers cryopreserved by an encapsulation/dehydration technique. In vitro Cell Dev Biol Plant 42:31–36

    Article  Google Scholar 

  • Mishra R, Rao GJN, Rao RN, Kaushal P (2015) Development and characterization of elite doubled haploid lines from two indica rice hybrids. Rice Sci 22:290–299

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for growth and bioassay with tobacco culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acid Res 19:4321–4325

    Article  Google Scholar 

  • Niroula RK, Bimb HP (2009) Effect of genotype and callus induction medium on green plant regeneration from anther of Nepalese rice cultivars. Asian J Plant Sci 8:368–373

    Article  CAS  Google Scholar 

  • Ochatt S, Pech C, Grewal R, Conreux C, Lulsdorf M, Jacas L (2009) Abiotic stress enhances androgenesis from isolated microspores of some legume species (Fabaceae). J Plant Physiol 166:1314–1328

    Article  CAS  PubMed  Google Scholar 

  • Raina SK, Zapata FJ (1997) Enhanced anther culture efficiency of indica rice (Oryza sativa L.) through modification of the culture media. Plant Breed 116:305–315

    Article  CAS  Google Scholar 

  • Roberts-Oehlschlager SL, Dunwell JM, Faulks R (1990) Changes in sugar content of barley anthers during culture on different carbohydrates. Plant Cell Tissue Organ Cult 22:77–85

    Article  CAS  Google Scholar 

  • SAS Institute (2003) The SAS system for Windows. Release 9.1.3. SAS Institute, Cary

    Google Scholar 

  • Senadhira D, Zapata-Arias FJ, Gregorio GB, Alejar MS, De La Cruz HC, Padolina TF, Galvez AM (2002) Development of the first salt tolerant rice cultivar through indica/indica anther culture. Field Crops Res 76:103–110

    Article  Google Scholar 

  • Shahvali-Kohshour R, Moieni A, Baghizadeh A (2013) Positive effects of cold pretreatment, iron source, and silver nitrate on anther culture of strawberry (Fragaria × Ananassa Duch.). Plant Biotechnol Rep 7:481–488

    Article  Google Scholar 

  • Shariatpanahi ME, Bal U, Heberle-Bors E, Touraev A (2006) Stresses applied for the re-programming of plant microspores towards in vitro embryogenesis. Physiol Plant 127:519–534

    Article  CAS  Google Scholar 

  • Sripichitt P, Ozawa T, Otani M, Shimada T (2000) Improved method for anther culture of an indica rice cultivar of Thailand. Plant Prod Sci 3:254–256

    Article  Google Scholar 

  • Suhartini T, Hanarida I (2000) Genetic similarity of rice lines resulted from F-1 anther culture of H1 generation. Pen Tan Pang 19:13–20

    Google Scholar 

  • Suriyan C, Bootsaya S, Aussanee P, Chalermpol K (2009) An efficient procedure for embryogenic callus induction and doubled haploid plant regeneration through anther culture of Thai aromatic rice (Oryza sativa L. subsp. indica). In vitro Cell Dev Biol Plant 45:171–179

    Article  Google Scholar 

  • Uno Y, Koda-Katayama H, Kobayashi H (2016) Application of anther culture for efficient haploid production in the genus Saintpaulia. Plant Cell Tissue Organ Cult 125:241–248

    Article  CAS  Google Scholar 

  • Yuan LP (1994) Increasing yield potential in rice by exploitation of heterosis. In: Virmani SS (ed) Hybrid rice technology: new developments and future prospects. IRRI, Manila, pp 1–6

    Google Scholar 

  • Zapata FJ, Alejar MS, Torrizo LB, Novero AY, Singh UP, Senadhira D (1991) Field performance of anther culture-derived lines from F1 cross of indica rice under saline and non-saline conditions. Theor Appl Genet 83:6–11

    Article  CAS  PubMed  Google Scholar 

  • Zapata-Arias FJ (2003) Laboratory protocol for anther culture technique in rice. In: Maluszynski M, Kasha KJ, Forster BP, Szarejko I (eds) Double haploid production in crop plants: a manual, Kluwer, Dordrecht, pp 109–116

    Chapter  Google Scholar 

Download references

Acknowledgements

The authors wish to acknowledge the help of the National Rice Research Institute (formerly CRRI), Cuttack, Odisha for providing necessary facilities and also to M/S Bayer Seeds, Hyderabad, Telangana for supplying the F1 seeds of BS6444G.

Author information

Authors and Affiliations

  1. Crop Improvement Division, ICAR-National Rice Research Institute, Cuttack, Odisha, 753 006, India

    Nupur Naik, Prachitara Rout, Ngangkham Umakanta, Ram Lakhan Verma, Jawahar Lal Katara, Onkar Nath Singh & Sanghamitra Samantaray

  2. Ravenshaw University, Cuttack, Odisha, India

    Khirod Kumar Sahoo

Authors
  1. Nupur Naik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Prachitara Rout
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ngangkham Umakanta
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ram Lakhan Verma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jawahar Lal Katara
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Khirod Kumar Sahoo
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Onkar Nath Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Sanghamitra Samantaray
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Sanghamitra Samantaray.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s11240-016-1163-6.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Naik, N., Rout, P., Umakanta, N. et al. Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture. Plant Cell Tiss Organ Cult 128, 679–689 (2017). https://doi.org/10.1007/s11240-016-1149-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-016-1149-4

Keywords

Search

Navigation