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Nephrocystidium pickii Weissenberg, 1921 belongs to Myxozoa (Cnidaria) but is not conspecific with Myxidium lieberkuehni Bütschli, 1882 (Myxozoa: Bivalvulida: Variisporina: Myxidiidae): molecular-genetic evidence

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Abstract

We isolated and re-investigated Nephrocystidium pickii Weißenberg, 1921 (Myxozoa: Bivalvulida: Variisporina) using light microscopy and phylogenetic analysis of the small-subunit (SSU) ribosomal RNA gene. This species is a parasite of the northern pike Esox lucius L. (Actinopterygii: Esocidae) which localizes in the endothelial cells of the glomerular capillary. The results of the phylogenetic analysis including this species clarify its taxonomic status and show that although it is the closest relative to Myxidium lieberkuehni Bütschli, 1882, the two organisms are not conspecific, contrary to the earlier hypotheses. The data obtained highlight the necessity of a profound taxonomic revision of the Myxozoa and the need to clarify species affiliation of extrasporogonic developmental stages of these organisms that occur in different organs of fish.

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Acknowledgements

The study utilized the equipment of the Core Facility Centres “Development of Molecular and Cell Technologies”, “Culture Collection of Microorganisms” of the St. Petersburg State University Research Park, and “Karelian Research Centre of the Russian Academy of Sciences”. The authors wish to thank S. A. Murzina (IB KarRC RAS, Petrozavodsk) for help in making histology sections.

Funding

This research was performed with the financial support of the “Bioresources” programme of the Russian Academy of Sciences and within the framework of the budgetary themes №№ 0221-2017-0042 and AAAA-A17-117030310322-3.

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Authors and Affiliations

  1. A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia

    Sergey Sokolov

  2. Institute of Biology of the Karelian Research Centre of the Russian Academy of Sciences, Petrozavodsk, Russia

    Sergey Sokolov & Aleksey Parshukov

  3. Department of Invertebrate Zoology, Faculty of Biology, St. Petersburg State University, St. Petersburg, Russia

    Ekaterina Volkova & Alexander Kudryavtsev

  4. Core Facility Centre “Culture Collection of Microorganisms”, St. Petersburg State University Research Park, St. Petersburg, Russia

    Ekaterina Volkova

  5. Laboratory of Parasitic Worms and Protistology, Zoological Institute, Russian Academy of Sciences, St. Petersburg, Russia

    Ekaterina Volkova & Alexander Kudryavtsev

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  1. Sergey Sokolov
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  2. Ekaterina Volkova
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Correspondence to Aleksey Parshukov.

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Sokolov, S., Volkova, E., Kudryavtsev, A. et al. Nephrocystidium pickii Weissenberg, 1921 belongs to Myxozoa (Cnidaria) but is not conspecific with Myxidium lieberkuehni Bütschli, 1882 (Myxozoa: Bivalvulida: Variisporina: Myxidiidae): molecular-genetic evidence. Syst Parasitol 96, 15–22 (2019). https://doi.org/10.1007/s11230-018-9834-9

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