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Pharyngostrongylus thylogale n. sp. (Nematoda: Strongylida) from the stomachs of macropodid marsupials defined by morphological and molecular criteria

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Abstract

Pharyngostrongylus thylogale n. sp. (Nematoda: Strongylida) is described from the stomach of the red-legged pademelon, Thylogale stigmatica (Gould) (Marsupialia: Macropodidae) from north-eastern Queensland and Papua New Guinea, having formerly been confused with P. iota Johnston & Mawson, 1939. Pharyngostrongylus thylogale n. sp. differs from all congeners in having 12 labial crown elements rather than eight or 16. Pharyngostrongylus iota was found in T. stigmatica, but only in southern Queensland and northern New South Wales, in the subspecies T. s. wilcoxi, compared with P. thylogale n. sp. which was found in T. s. stigmatica in northern Queensland and T. s. oriomo in Papua New Guinea. Differences in the sequences of the first and second internal transcribed spacers (ITS-1 and ITS-2) of the nuclear ribosomal DNA of P. thylogale n. sp. and ten congeners support the erection of the new species, and the validity of the morphospecies examined. However, results of the phylogenetic analyses of the molecular data also provide evidence for the existence of cryptic species within P. kappa Mawson, 1965. No obvious co-evolutionary relationships were observed between parasite species and their macropodid marsupial hosts.

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References

  • Aussavy, M., Bernardin, E., Corrigan, A., Hufschmid, J., & Beveridge, I. (2011). Helminth parasite communities in four species of sympatric macropodids in western Victoria. Australian Mammalogy, 33, 13–20.

    Article  Google Scholar 

  • Beveridge, I. (1982). A taxonomic revision of the Pharyngostrongylinea Popova (Nematoda: Strongyloidea) from macropodid marsupials. Australian Journal of Zoology, Supplementary Series, 83, 1–150.

    Article  Google Scholar 

  • Beveridge, I., & Chilton, N. B. (2001). Co-evolutionary relationships between the nematode subfamily Cloacininae and its macropodid marsupial hosts. International Journal for Parasitology, 31, 976–996.

    Article  CAS  PubMed  Google Scholar 

  • Beveridge, I., Chilton, N. B., Johnson, P. M., Smales, L. R., Speare, R., & Spratt, D. M. (1998). Helminth parasite communities of kangaroos and wallabies (Macropus spp. and Wallabia bicolor) from north and central Queensland. Australian Journal of Zoology, 46, 473–495.

    Article  Google Scholar 

  • Beveridge, I., & Gasser, R. B. (2014). Diversity in parasitic helminths of Australasian marsupials and monotremes: a molecular perspective. International Journal for Parasitology, 44, 859–864.

    Article  PubMed  Google Scholar 

  • Beveridge, I., Speare, R., Johnson, P. M., & Spratt, D. M. (1992). Helminth parasite communities of macropodoid marsupials of the genera Hypsiprymnodon, Aepyprymnus, Thylogale, Onychogale, Lagorchestes and Dendrolagus from Queensland. Wildlife Research, 19, 359–376.

    Article  Google Scholar 

  • Beveridge, I., Spratt, D. M., Close, R. L., Barker, S. C., & Sharman, G. B. (1989). Helminth parasites of rock wallabies, Petrogale spp. (Marsupialia) from Queensland. Australian Wildlife Research, 16, 273–287.

    Article  Google Scholar 

  • Cardillo, M., Bininda-Edmonds, O. R. P., Boakes, E., & Purvis, A. (2004). A species-level phylogenetic supertree of marsupials. Journal of Zoology (London), 264, 11–31.

    Article  Google Scholar 

  • Eldridge, M. D. B., Heckengerg, K., Neaves, L. E., Metcalfe, C. J., Hamilton, S., Johnson, P. M., et al. (2011). Genetic differentiation and introgression amongst Thylogale (pademelons) taxa in eastern Australia. Australian Journal of Zoology, 59, 103–117.

    Article  Google Scholar 

  • Gasser, R. B., Chilton, N. B., Hoste, H., & Beveridge, I. (1993). Rapid sequencing of rDNA from single worms and eggs of parasitic helminths. Nucleic Acids Research, 21, 2525–2526.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Griffith, J. E., Beveridge, I., Chilton, N. B., & Johnson, P. M. (2000). Helminth communities of pademelons, Thylogale stigmatica and T. thetis (Marsupialia: Macropodidae) from eastern Australia and Papua New Guinea. Journal of Helminthology, 74, 307–314.

    CAS  PubMed  Google Scholar 

  • Lichtenfels, J. R. (1980). Keys to genera of the superfamily Strongyloidea No. 7. In R. C. Anderson, A. G. Chabaud, & S. Willmott (Eds.), CIH Keys to the nematode parasites of vertebrates. Farnham Royal: Commonwealth Agricultural Bureaux, 41 pp.

  • Meredith, R. W., Westerman, M., & Springer, M. S. (2008). A phylogeny and timescale for the living genera of kangaroos and kin (Macropodiformes: Marsupialia) based on nuclear DNA sequences. Australian Journal of Zoology, 56, 395–410.

    Article  CAS  Google Scholar 

  • Posada, D. (2008). jModelTest: phylogenetic model averaging. Molecular and Biological Evolution, 25, 1253–1256.

    Article  CAS  Google Scholar 

  • Ronquist, F., & Huelsenbeck, J. P. (2003). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19, 1572–1574.

    Article  CAS  PubMed  Google Scholar 

  • Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., et al. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science, 239, 487–491.

    Article  CAS  PubMed  Google Scholar 

  • Spratt, D. M., & Beveridge, I. (2016). The helminth parasites of Australasian monotremes and marsupials. Zootaxa, 4123, 1–198.

    Article  PubMed  Google Scholar 

  • Swofford, D. L. (1999). PAUP*4.0b10. Sutherland: Sinauer Associates.

    Google Scholar 

  • van Dyck, S., & Strahan, R. (Eds.). (2008). The Mammals of Australia (3rd ed.). Chatswood, Australia: Reed New Holland Publishers, 887 pp.

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Acknowledgements

We wish to thank Dr Ifor Owen for collecting the specimens from Papua New Guinea.

Funding

This study was supported by funding from the Australian Research Council and the Australian Biological Resources Study.

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Authors and Affiliations

  1. Department of Biology, University of Saskatchewan, Saskatoon, SK, Canada

    Neil B. Chilton

  2. Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, VIC, Australia

    Florence Huby-Chilton, Robin B. Gasser, Anson V. Koehler & Ian Beveridge

Authors
  1. Neil B. Chilton
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  2. Florence Huby-Chilton
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  3. Robin B. Gasser
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  4. Anson V. Koehler
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  5. Ian Beveridge
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Corresponding author

Correspondence to Ian Beveridge.

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The authors declare that they have no conflict of interest.

Ethical approval

All material was collected under appropriate permits. Permits to collect specimens were provided by the National Parks & Wildlife Service of New South Wales (A681) and the Forestry Commission of New South Wales (03577), the Queensland Department of Environment & Conservation (T00436), the Queensland Department of Environment & Heritage (T00759, T00943) and the Northern Territory Department of Primary Industry (15747).

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Chilton, N.B., Huby-Chilton, F., Gasser, R.B. et al. Pharyngostrongylus thylogale n. sp. (Nematoda: Strongylida) from the stomachs of macropodid marsupials defined by morphological and molecular criteria. Syst Parasitol 93, 749–760 (2016). https://doi.org/10.1007/s11230-016-9661-9

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  • DOI: https://doi.org/10.1007/s11230-016-9661-9

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