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Synthesis of a conjugate of 3´-sialyllactoside with recombinant flagellin as a carrier protein and assessment of its immunological activity in comparison with that of a similar hemocyanin-based conjugate

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Abstract

In the framework of a project directed to the design of synthetic cancer vaccines based on carbohydrate chains of tumor-associated gangliosides, a new prototype cancer vaccine, a polyvalent carbohydrate-protein conjugate 1 with ∼11 3´-sialyllactoside ligands conjugated to one molecule of recombinant flagellin was synthesized. Dendritic cell vaccines were designed using the conjugate 1 and a neoglycoconjugate 2 consisting of ∼400 3´-sialyllactose residues linked to hemocyanin from a snail Megathura crenulata (KLH). Comparative ELISA study was conducted to assess the induction of carbohydrate-specific immunoglobulins IgM and IgG after immunization of mice with conjugates 1 and 2 and dendritic cells loaded with 1 and 2. Synthetic polyacrylamide carrier conjugated to 3´-sialyllactose ligands and B16F0 melanoma cells were used as coating antigens.

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Authors and Affiliations

  1. National Research Center “Institute of Immunology”, Federal Medical-Biological Agency of Russia, 24/2, Kashirskoe sh., 115478, Moscow, Russian Federation

    E. M. Novikova, N. A. Razvalyaeva & R. N. Stepanenko

  2. N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 47 Leninskii prosp., 119991, Moscow, Russian Federation

    E. A. Khatuntseva, Yu. E. Tsvetkov & N. E. Nifantiev

  3. Scientific and Technological Center “BioInvest”, 20/3 Nauchnii pr-d, 117246, Moscow, Russian Federation

    D. A. Goncharuk & O. A. Zeynalov

Authors
  1. E. M. Novikova
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  2. E. A. Khatuntseva
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  3. Yu. E. Tsvetkov
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  4. N. A. Razvalyaeva
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  5. D. A. Goncharuk
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  6. O. A. Zeynalov
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  7. N. E. Nifantiev
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  8. R. N. Stepanenko
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Corresponding author

Correspondence to R. N. Stepanenko.

Additional information

Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 7, pp. 1640–1647, July, 2015.

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Novikova, E.M., Khatuntseva, E.A., Tsvetkov, Y.E. et al. Synthesis of a conjugate of 3´-sialyllactoside with recombinant flagellin as a carrier protein and assessment of its immunological activity in comparison with that of a similar hemocyanin-based conjugate. Russ Chem Bull 64, 1640–1647 (2015). https://doi.org/10.1007/s11172-015-1054-8

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  • DOI: https://doi.org/10.1007/s11172-015-1054-8

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