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In vitro antimicrobial evaluation, effects of halide concentration and hemolysis study of silver-N-heterocyclic carbene complexes

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Abstract

The morpholine containing silver complexes were screened for the zone of inhibition, MIC determination, and longevity studies against bacterial growth. The most potent compounds SC-4 (Silver Complex-4) and SC-5 inhibited the bacterial growth with MIC of 12.5 and 6.25 µM against Staphylococcus aureus, respectively, and 50 µM against Salmonella enterica with an efficient growth inhibition up to 21 days with disruption of cell wall as monitored by SEM analysis. The side chain was varied to evaluate the possible changes in antimicrobial effects. The Ag–NHCs SC-4 and SC-5 had shown enhanced bactericidal effects due to the synergistic effects of NaCl and Ag–NHCs when studied at 12.5 µM along with 10 g/L NaCl. The complex SC-5 induced considerable RBC (red blood corpuscles) rupture at 50 µM compared to IS-5 (imidazolium salt) and directly proportional to concentration.

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Acknowledgements

We gratefully acknowledge SIF DST-VIT-FIST Vellore for providing NMR, FT-IR data; “Smart Materials Laboratory for Biosensing and Catalysis” for providing excellent research facilities, and also the SEM facility at SBST, RGEMS, VIT, Vellore for funding the research work. The authors are highly thankful to CDRI, Lucknow for providing CHNO data and SAIF, IIT Madras for HR-MS (EI) analysis.

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Authors and Affiliations

  1. Department of Chemistry, School of Advanced Sciences, VIT, Vellore, Tamilnadu, 632014, India

    Sainath Aher & Pundlik Bhagat

  2. Department of Biosciences, School of Biosciences and Biotechnology, VIT, Vellore, Tamilnadu, 632014, India

    Anamika Das & Jabez Osborne

  3. Department of Chemistry, Amlokchand Mahavidyalaya Yavatmal, Sant Gadge Baba Amravati University, Amravati, 445001, India

    Prashant Muskawar

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  1. Sainath Aher
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  5. Pundlik Bhagat
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Correspondence to Pundlik Bhagat.

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Aher, S., Das, A., Muskawar, P. et al. In vitro antimicrobial evaluation, effects of halide concentration and hemolysis study of silver-N-heterocyclic carbene complexes. Res Chem Intermed 44, 2099–2110 (2018). https://doi.org/10.1007/s11164-017-3216-9

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  • DOI: https://doi.org/10.1007/s11164-017-3216-9

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