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Modulation Mechanism of Wuniuzao Dark Tea Polysaccharide on Lipid Metabolism in Hyperlipidemic Mice Induced by High-Fat Diet

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Abstract

The aim of this study was to investigate the functional mechanism of Wuniuzao dark tea polysaccharide (WDTP) that protect against hyperlipidemia in mice induced by high-fat diet. WDTP was extracted by hot water, isolated and purified by DEAE-52 chromatography and characterized by high-performance liquid chromatograph (HPLC), Fourier transform infrared spectroscopy (FT-IR) and scanning electron microscope (SEM). Different doses (200 or 800 mg/kg/day) of WDTP were orally administered to mice induced by high-fat diet to evaluate the mechanism of WDTP regulating lipid metabolism. And these results showed that average molecular weight of WDTP was nearly 63,869 Da. And WDTP intervention significantly reduced body weight, lipid accumulation, and modulated blood lipid levels. The mechanism of WDTP ameliorating lipid metabolism was associated with regulating the expression of lipid metabolism-related genes and serum exosomes miR-19b-3p, and modulating the community structure of gut microbiota in mice.

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Data Availability

Raw sequence data from all 16 S rRNA sequencing experiments in this study have been deposited in the GSA (https://ngdc.cncb.ac.cn/gsa/browse/) under accession number CRA012322.

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Acknowledgements

We are grateful to thank Luting Ye, Guangmei Li, Shengnan Zhu, Weijia Xu, Zhaowen Chen, Simin Ren, Min Cheng and Jingwu Song (College of Life Sciences, China Jiliang University) for generously providing rats administration. Financial assistance from the National Natural Science Foundation of China and the Major Science and Technology Projects in Zhejiang Province are sincerely thanked.

Funding

This work was supported financially by the National Natural Science Foundation of China (31100499 and 31672394), the Major Science and Technology Projects in Zhejiang Province (2020C02045) and Zhejiang Science and Technology Commissioner Team Project.

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Author notes

  1. Xuanxuan Zou and Zonghua Dong contributed equally to this work.

Authors and Affiliations

  1. College of Life Sciences, China Jiliang University, Hangzhou, Zhejiang, 310018, China

    Xuanxuan Zou, Zonghua Dong, Jin Zhao, Feng Guan & Jian Ge

  2. Institute of Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang, 310021, China

    Wei Liu

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  1. Xuanxuan Zou
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Contributions

X. Z., Z. D. and J. G. conceived the project. X. Z. and Z. D. analyzed the data. X. Z. and Z. D. wrote the manuscript. X. Z., Z. D., W. L., J. Z., F. G. and J. G. provided critical discussion, editing and final approval of the manuscript. All authors reviewed the manuscript.

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Correspondence to Jian Ge.

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Ethical Approval

The animal experiments protocol was approved according to the agreement of Laboratory Animal Ethics Committee from China Jiliang University (2022-003), and all animal procedures were performed in accordance with the institutional and national guidelines.

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All authors declare that they have known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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Zou, X., Dong, Z., Liu, W. et al. Modulation Mechanism of Wuniuzao Dark Tea Polysaccharide on Lipid Metabolism in Hyperlipidemic Mice Induced by High-Fat Diet. Plant Foods Hum Nutr 79, 173–181 (2024). https://doi.org/10.1007/s11130-024-01145-2

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