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Sorghum Phenolics Inhibits Inflammasomes in Lipopolysaccharide (LPS)-Primed and Adenosine Triphosphate (ATP)-Activated Macrophages

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Abstract

Sorghum contains phenolic compounds with reported biological activities. The objective was to evaluate the ability of sorghum phenolic extract to inhibit inflammasomes in THP-1 human macrophages. THP-1 human macrophages was pre-treated with sorghum phenolics and the inflammasome was activated by lipopolysaccharide and adenosine triphosphate treatment. Treatment of macrophages with 50 μg sorghum extract/mL reduced IL-1β and IL-18 secretion by 59.7 and 32.0%, respectively, associated with caspase-1 activity reduction. Moreover, the production of intracellular reactive oxygen species was reduced. Our data showed the potential role of sorghum phenolics in diseases associated with aberrant inflammasomes activation.

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Abbreviations

ASC:

apoptosis-associated speck-like protein containing a caspase-recruitment domain

ATP:

adenosine triphosphate

FBS:

fetal bovine serum

LPS:

lipopolysaccharide

NLRP:

nucleotide-binding and oligomerization domain-like and pyrin

PMA:

phorbol 12-myristate 13-acetate

RIPA:

radioimmunoprecipitation assay

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Acknowledgements

This work is supported by the USDA National Institute of Food and Agriculture, HATCH 1010230 to VPD.

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Authors and Affiliations

  1. Department of Food Science, The University of Tennessee, Knoxville, TN, USA

    Vermont P. Dia, Jordan Bradwell & Philipus Pangloli

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  1. Vermont P. Dia
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Correspondence to Vermont P. Dia.

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Dia, V.P., Bradwell, J. & Pangloli, P. Sorghum Phenolics Inhibits Inflammasomes in Lipopolysaccharide (LPS)-Primed and Adenosine Triphosphate (ATP)-Activated Macrophages. Plant Foods Hum Nutr 74, 307–315 (2019). https://doi.org/10.1007/s11130-019-00736-8

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