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Comprehensive Evaluation of Antioxidant Potential of 10 Salvia Species Using High Pressure Methods for the Isolation of Lipophilic and Hydrophilic Plant Fractions

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Abstract

Common sage (Salvia officinalis) is a well-known source of antioxidants and other bioactive compounds, while many other species within the Salvia genus have been poorly studied. The total content of phenolic compounds (TPC) and antioxidant capacity indicators were evaluated for the extracts of 10 Salvia spp. consecutively isolated by supercritical carbon dioxide (SFE-CO2) and pressurized liquid extraction with ethanol and water. Antioxidant properties of solid plant material were evaluated by the direct antioxidant capacity measurement by the so-called QUENCHER method. Total antioxidant capacity values were calculated by integrating the results obtained for all extracts and the whole plant material. TPC and antioxidant capacity of the extracts were greatly dependent on the plant species and extraction solvent. Ethanol extracts possessed significantly higher antioxidant capacity and TPC comparing to the extracts isolated with other solvents. In general, all studied Salvia species demonstrated strong antioxidant capacity; however, the antioxidant potential of such species as S. forsskaolii and S. verticillata was the highest and comparable with that of S. officinalis. The majority of studied Salvia species may be considered as promising sources of functional ingredients to be used in human nutrition for functional food and nutraceutical formulations.

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Abbreviations

AAPH:

2,2-azobis-(2-amidino-propane) dihydrochloride

ABTS:

2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)

DWP:

Dry weight of plant

DWE:

Dry weight of extract

GAE:

Gallic acid equivalents

ORAC:

Oxygen radical absorbance capacity

PLE:

Pressurized liquid extraction

RSC:

Radical scavenging capacity

SFE:

Supercritical fluid extraction

TE:

Trolox equivalents

TEAC:

Trolox equivalent antioxidant capacity

TPC:

Total phenolic content

Trolox:

6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid

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Acknowledgments

This study was supported by the Research Council of Lithuania and National Paying Agency under the Ministry of Agriculture of The Republic of Lithuania (project MT-1131).

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Authors and Affiliations

  1. Department of Food Science and Technology, Kaunas University of Technology, Radvilėnų pl. 19, Kaunas, LT-50254, Lithuania

    Vaida Šulniūtė & Petras Rimantas Venskutonis

  2. Kaunas Botanical Garden, Vytautas Magnus University, Žilibero 6, LT-46324, Kaunas, Lithuania

    Ona Ragažinskienė

Authors
  1. Vaida Šulniūtė
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  2. Ona Ragažinskienė
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  3. Petras Rimantas Venskutonis
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Correspondence to Petras Rimantas Venskutonis.

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Šulniūtė, V., Ragažinskienė, O. & Venskutonis, P.R. Comprehensive Evaluation of Antioxidant Potential of 10 Salvia Species Using High Pressure Methods for the Isolation of Lipophilic and Hydrophilic Plant Fractions. Plant Foods Hum Nutr 71, 64–71 (2016). https://doi.org/10.1007/s11130-015-0526-1

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