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Light-harvesting complex stress-related proteins play crucial roles in the acclimation of Physcomitrella patens under fluctuating light conditions

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Abstract

Photosynthetic organisms have evolved photoprotective mechanisms to acclimate to light intensity fluctuations in their natural growth environments. Photosystem (PS) II subunit S (PsbS) and light-harvesting complex (LHC) stress-related proteins (LhcSR) are essential for triggering photoprotection in vascular plants and green algae, respectively. The activity of both proteins is strongly enhanced in the moss Physcomitrella patens under high-light conditions. However, their role in regulating photosynthesis acclimation in P. patens under fluctuating light (FL) conditions is still unknown. Here, we compare the responses of wild-type (WT) P. patens and mutants lacking PsbS (psbs KO) or LhcSR1 and 2 (lhcsr KO) to FL conditions in which the low-light phases were periodically interrupted with high-light pulses. lhcsr KO mutant showed a strong reduction in growth with respect to WT and psbs KO under FL conditions. The lack of LhcSR not only decreased the level of non-photochemical quenching, resulting in an over-reduced plastoquinone pool, but also significantly increased the PSI acceptor limitation values with respect to WT and psbs KO under FL conditions. Moreover, in lhcsr KO mutant, the abundance of PSI core and PSI–LHCI complex decreased greatly under FL conditions compared with the WT and psbs KO. We proposed that LhcSR in P. patens play a crucial role in moss acclimation to dynamic light changes.

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Acknowledgements

The authors have no conflict of interest. This study was supported by National Natural Science Foundation of China (No. 41976097), the National Key R&D Program of China (2018YFD0901500), the Key Deployment Project of the Centre for Ocean Mega-Research of Science, the Chinese Academy of Sciences (COMS2019Q02), the Major Scientific and Technological Innovation Projects in Shandong Province (2019JZZY010815), China Agriculture Research System of MOF and MARA (CARS-50), the North Jiangsu Science and Technology Special Project (LYG-SZ201916), and the Science and technology Project of Dongtou District (N2016Y18A).

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  1. Zhuangyue Li

    Present address: Department of Biology, College of Arts and Sciences, Case Western Reserve University, Cleveland, USA

Authors and Affiliations

  1. CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China

    Shan Gao, Lu Zhou, Zhenbing Zheng, Zhuangyue Li & Guangce Wang

  2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China

    Shan Gao, Lu Zhou, Zhenbing Zheng & Guangce Wang

  3. Department of Biology and Biotechnology ‘L. Spallanzani’(DBB), University of Pavia, Pavia, Italy

    Alberta Pinnola

  4. College of Earth Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China

    Lu Zhou

  5. Department of Biotechnology, University of Verona, Verona, Italy

    Roberto Bassi

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  1. Shan Gao
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Correspondence to Guangce Wang.

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Gao, S., Pinnola, A., Zhou, L. et al. Light-harvesting complex stress-related proteins play crucial roles in the acclimation of Physcomitrella patens under fluctuating light conditions. Photosynth Res 151, 1–10 (2022). https://doi.org/10.1007/s11120-021-00874-8

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  • DOI: https://doi.org/10.1007/s11120-021-00874-8

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