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Adaptation of light-harvesting and energy-transfer processes of a diatom Phaeodactylum tricornutum to different light qualities

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Abstract

Fucoxanthin-chlorophyll (Chl) a/c–binding proteins (FCPs) are light-harvesting pigment–protein complexes found in diatoms and brown algae. Due to the characteristic pigments, such as fucoxanthin and Chl c, FCPs can capture light energy in blue-to green regions. A pennate diatom Phaeodactylum tricornutum synthesizes a red-shifted form of FCP under weak or red light, extending a light-absorption ability to longer wavelengths. In the present study, we examined changes in light-harvesting and energy-transfer processes of P. tricornutum cells grown under white- and single-colored light-emitting diodes (LEDs). The red-shifted FCP appears in the cells grown under the green, yellow, and red LEDs, and exhibited a fluorescence peak around 714 nm. Additional energy-transfer pathways are established in the red-shifted FCP; two forms (F713 and F718) of low-energy Chl a work as energy traps at 77 K. Averaged fluorescence lifetimes are prolonged in the cells grown under the yellow and red LEDs, whereas they are shortened in the blue-LED-grown cells. Based on these results, we discussed the light-adaptation machinery of P. tricornutum cells involved in the red-shifted FCP.

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Abbreviations

AFDA:

Absolute fluorescence decay-associated

Car:

Carotenoid

Chl:

Chlorophyll

FCP:

Fucoxanthin Chl a/c-binding protein

FDA:

Fluorescence decay-associated

LED:

Light-emitting diode

LHC:

Light-harvesting complex

PS:

Photosystem

RC:

Reaction center

TRF:

Time-resolved fluorescence

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Acknowledgements

This work was supported by the Grants-in-Aid for Scientific Research from Japan Society for the Promotion of Science KAKENHI JP18J10095 (to Y.U.), JP17H06433 (to J.-R.S.), JP17K07442 and JP19H04726 (to R.N.), and JP16H06553 (to S.A.).

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Authors and Affiliations

  1. Faculty of Science, Kobe University, Kobe, 657-8501, Japan

    Kumiko Oka & Seiji Akimoto

  2. Graduate School of Science, Kobe University, Kobe, 657-8501, Japan

    Yoshifumi Ueno & Seiji Akimoto

  3. Innovation Center, Nippon Flour Mills Co., Ltd, Atsugi, 243-0041, Japan

    Makio Yokono

  4. Research Institute for Interdisciplinary Science and Graduate School of Natural Science and Technology, Okayama University, Okayama, 700-8530, Japan

    Jian-Ren Shen & Ryo Nagao

Authors
  1. Kumiko Oka
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  2. Yoshifumi Ueno
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  3. Makio Yokono
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  4. Jian-Ren Shen
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  5. Ryo Nagao
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  6. Seiji Akimoto
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Correspondence to Ryo Nagao or Seiji Akimoto.

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Oka, K., Ueno, Y., Yokono, M. et al. Adaptation of light-harvesting and energy-transfer processes of a diatom Phaeodactylum tricornutum to different light qualities. Photosynth Res 146, 227–234 (2020). https://doi.org/10.1007/s11120-020-00714-1

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