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Similar photosynthetic response to elevated carbon dioxide concentration in species with different phloem loading strategies

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Abstract

Species have different strategies for loading sugars into the phloem, which vary in the route that sugars take to enter the phloem and the energetics of sugar accumulation. Species with passive phloem loading are hypothesized to have less flexibility in response to changes in some environmental conditions because sucrose export from mesophyll cells is dependent on fixed anatomical plasmodesmatal connections. Passive phloem loaders also have high mesophyll sugar content, and may be less likely to exhibit sugar-mediated down-regulation of photosynthetic capacity at elevated CO2 concentrations. To date, the effect of phloem loading strategy on the response of plant carbon metabolism to rising atmospheric CO2 concentrations is unclear, despite the widespread impacts of rising CO2 on plants. Over three field seasons, five species with apoplastic loading, passive loading, or polymer-trapping were grown at ambient and elevated CO2 concentration in free air concentration enrichment plots. Light-saturated rate of photosynthesis, photosynthetic capacity, leaf carbohydrate content, and anatomy were measured and compared among the species. All five species showed significant stimulation in midday photosynthetic CO2 uptake by elevated CO2 even though the two passive loading species showed significant down-regulation of maximum Rubisco carboxylation capacity at elevated CO2. There was a trend toward greater starch accumulation at elevated CO2 in all species, and was most pronounced in passive loaders. From this study, we cannot conclude that phloem loading strategy is a key determinant of plant response to elevated CO2, but compelling differences in response counter to our hypothesis were observed. A phylogenetically controlled experiment with more species may be needed to fully test the hypothesis.

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Acknowledgements

This work was supported by funding from the United States Department of Agriculture, National Institute for Food & Agriculture Grant No. 2015-67013-22836. We thank Matt Lyons for assistance with field work and Alex Ulanov for assistance with metabolite analysis.

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  1. Kristen A. Bishop and Pauline Lemonnier have equally contributed to this work.

Authors and Affiliations

  1. Departments of Plant Biology and Crop Sciences and Institute for Genomic Biology, University of Illinois at Urbana–Champaign, Champaign, USA

    Kristen A. Bishop, Pauline Lemonnier, Jennifer C. Quebedeaux, Christopher M. Montes, Andrew D. B. Leakey & Elizabeth A. Ainsworth

  2. USDA ARS Global Change and Photosynthesis Research Unit, 1201 W. Gregory Drive, Urbana, IL, 61801, USA

    Pauline Lemonnier & Elizabeth A. Ainsworth

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  1. Kristen A. Bishop
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  2. Pauline Lemonnier
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Correspondence to Elizabeth A. Ainsworth.

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Bishop, K.A., Lemonnier, P., Quebedeaux, J.C. et al. Similar photosynthetic response to elevated carbon dioxide concentration in species with different phloem loading strategies. Photosynth Res 137, 453–464 (2018). https://doi.org/10.1007/s11120-018-0524-x

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