Abstract
Far-Red Light (FRL) acclimation is a process that has been observed in cyanobacteria and algae that can grow solely on light above 700 nm. The acclimation to FRL results in rearrangement and synthesis of new pigments and pigment-protein complexes. In this study, cyanobacteria containing chlorophyll f, Synechococcus sp. PCC 7335 and Halomicronema hongdechloris, were imaged as live cells with confocal microscopy. H. hongdechloris was further studied with hyperspectral confocal fluorescence microscopy (HCFM) and freeze-substituted thin-section transmission electron microscopy (TEM). Under FRL, phycocyanin-containing complexes and chlorophyll-containing complexes were determined to be physically separated and the synthesis of red-form phycobilisome and Chl f was increased. The timing of these responses was observed. The heterogeneity and eco-physiological response of the cells was noted. Additionally, a gliding motility for H. hongdechloris is reported.
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Abbreviations
- 1D:
-
One day
- 3W:
-
Three weeks
- APC:
-
Allophycocyanin
- Chl:
-
Chlorophyll
- FaRLiP:
-
Far-red light photoacclimation
- FRL:
-
Far-red light
- H. hongdechloris :
-
Halomicronema hongdechloris
- HCFM:
-
Hyperspectral confocal fluorescence microscopy
- MCR:
-
Multivariate curve resolution
- PBS:
-
Phycobilisome
- PC:
-
Phycocyanin
- PS:
-
Photosystem
- PSI:
-
Photosystem I
- PSII:
-
Photosystem II
- Syn 7335:
-
Synechococcus sp. PCC 7335
- TEM:
-
Transmission electron microscopy
- WL:
-
White light
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Acknowledgements
This work was supported as part of the Photosynthetic Antenna Research Center (PARC), an Energy Frontier Research Center funded by the U.S. Department of Energy, Office of Science, Basic Energy Sciences under Award #DE-SC0001035”. The authors are grateful to Michael Sinclair for the use and maintenance of the hyperspectral confocal fluorescence microscope, Stephen Anthony for the current implementation of the MCR analysis software used to deconvolve spectral components; Howland Jones, Mark Van Benthem, David Melgaard, Mike Keenan, and David Haaland for original development of the MCR algorithm and software. Sandia National Laboratories is a multi-mission laboratory managed and operated by National Technology and Engineering Solutions of Sandia, LLC, a wholly owned subsidiary of Honeywell International, Inc., for the U.S. Department of Energy’s National Nuclear Security Administration under contract DE-NA0003525. E.L.-W.M. acknowledges graduate fellowships Mr. and Mrs. Spencer T. Olin Fellowship for Women and the P.E.O. Scholar Award.
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Majumder, E.LW., Wolf, B.M., Liu, H. et al. Subcellular pigment distribution is altered under far-red light acclimation in cyanobacteria that contain chlorophyll f . Photosynth Res 134, 183–192 (2017). https://doi.org/10.1007/s11120-017-0428-1
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DOI: https://doi.org/10.1007/s11120-017-0428-1