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Multiple copies of the PsbQ protein in a cyanobacterial photosystem II assembly intermediate complex

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Abstract

Photosystem II (PSII) undergoes frequent damage owing to the demanding electron transfer chemistry it performs. To sustain photosynthetic activity, damaged PSII undergoes a complex repair cycle consisting of many transient intermediate complexes. By purifying PSII from the cyanobacterium Synechocystis sp. PCC 6803 using a histidine-tag on the PsbQ protein, a lumenal extrinsic subunit, a novel PSII assembly intermediate was isolated in addition to the mature PSII complex. This new complex, which we refer to as PSII-Q4, contained four copies of the PsbQ protein per PSII monomer, instead of the expected one copy. In addition, PSII-Q4 lacked two other lumenal extrinsic proteins, PsbU and PsbV, which are present in the mature PSII complex. We suggest that PSII-Q4 is a late PSII assembly intermediate that is formed just before the binding of PsbU and PsbV, and we incorporate these results into an updated model of PSII assembly.

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Acknowledgments

We thank Dr. Terry Bricker for the kind gift of the HT3 strain of Synechocystis 6803, and all members of the Pakrasi lab for collegial discussions. This work was supported by funding from the National Science Foundation (NSF-MCB0745611) to H.B.P.

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    Authors and Affiliations

    1. Department of Biology, CB1137, Washington University, 1 Brookings Drive, St. Louis, MO, 63130, USA

      Haijun Liu, Daniel A. Weisz & Himadri B. Pakrasi

    2. Department of Chemistry, Washington University, St. Louis, MO, 63130, USA

      Daniel A. Weisz

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    1. Haijun Liu
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    Correspondence to Himadri B. Pakrasi.

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    Haijun Liu and Daniel A. Weisz have contributed equally to this study.

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    Liu, H., Weisz, D.A. & Pakrasi, H.B. Multiple copies of the PsbQ protein in a cyanobacterial photosystem II assembly intermediate complex. Photosynth Res 126, 375–383 (2015). https://doi.org/10.1007/s11120-015-0123-z

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