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Carotenoid–chlorophyll coupling and fluorescence quenching in aggregated minor PSII proteins CP24 and CP29

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Abstract

It is known that aggregation of isolated light-harvesting complex II (LHCII) in solution results in high fluorescence quenching, reduced chlorophyll fluorescence lifetime, and increased electronic coupling of carotenoid (Car) S1 and chlorophyll (Chl) Qy states, as determined by two-photon studies. It has been suggested that this behavior of aggregated LHCII mimics aspects of non-photochemical quenching processes of higher plants and algae. However, several studies proposed that the minor photosystem II proteins CP24 and CP29 also play a significant role in regulation of photosynthesis. Therefore, we use a simple protocol that allows gradual aggregation also of CP24 and CP29. Similarly, as observed for LHCII, aggregation of CP24 and CP29 also leads to increasing fluorescence quenching and increasing electronic Car S1–Chl Qy coupling. Furthermore, a direct comparison of the three proteins revealed a significant higher electronic coupling in the two minor proteins already in the absence of any aggregation. These differences become even more prominent upon aggregation. A red-shift of the Qy absorption band known from LHCII aggregation was also observed for CP29 but not for CP24. We discuss possible implications of these results for the role of CP24 and CP29 as potential valves for excess excitation energy in the regulation of photosynthetic light harvesting.

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Acknowledgments

This work was generously supported by a grant of the German Science Foundation (DFG, WA1305/3-2) and by the Dutch organization for Scientific research, division Earth and Life Sciences (NWO-ALW) via a Vici grant to RC.

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Authors and Affiliations

  1. Department for Biophysical Chemistry, Institute for Physical and Theoretical Chemistry, Technische Universität Braunschweig, Hans-Sommer-Str. 10, 38106, Braunschweig, Germany

    Christoph-Peter Holleboom, Daniel Alexander Gacek, Pen-Nan Liao & Peter Jomo Walla

  2. Department of Physics and Astronomy, Faculty of Sciences, Vrije Universiteit Amsterdam and LaserLaB Amsterdam, De Boelelaan 1081, 1081 HV, Amsterdam, The Netherlands

    Marco Negretti & Roberta Croce

  3. AG Biomolecular Spectroscopy and Single-Molecule Detection, Max-Planck-Institute for Biophysical Chemistry, Am Faßberg 11, 37077, Göttingen, Germany

    Peter Jomo Walla

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  1. Christoph-Peter Holleboom
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  2. Daniel Alexander Gacek
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  3. Pen-Nan Liao
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  4. Marco Negretti
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Correspondence to Peter Jomo Walla.

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Holleboom, CP., Gacek, D.A., Liao, PN. et al. Carotenoid–chlorophyll coupling and fluorescence quenching in aggregated minor PSII proteins CP24 and CP29. Photosynth Res 124, 171–180 (2015). https://doi.org/10.1007/s11120-015-0113-1

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