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PyMPV17, the MPV17 Homolog of Pyropia yezoensis (Rhodophyta), Enhances Osmotic Stress Tolerance in Chlamydomonas

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Abstract

Pyropia yezoensis is the most cultivated marine red algae in East Asia and is desiccation tolerant. PyMPV17, a desiccation stress response gene isolated from P. yezoensis, shares significant sequence similarity with mouse MPV17. A yeast knock-out mutant for SYM1, an MPV17 ortholog, could not grow on ethanol-containing medium at 38°C. Expression of PyMPV17 in yeast sym1 cells complemented the ethanol growth defect at 38°C, which suggests that PyMPV17 is a functional ortholog of SYM1. PyMPV17-green fluorescent protein (GFP) fluorescence was detected in mitochondria and PyMPV17 transcription was upregulated under desiccation stress in P. yezoensis gametophytes. Transcription of the PyMPV17 gene also increased in response to H2O2 and abscisic acid treatments. When PyMPV17 was introduced into the green alga Chlamydomonas, transgenic cells grew better than control cells on agar plates containing mannitol. Total malondialdehyde content in the transgenic cells was lower than that in the control cells under stress conditions. These results suggest that PyMPV17 reduces oxidative damage and contributes to the mechanism underlying tolerance to osmotic stress in red algae.

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Acknowledgments

This study was supported by the Korean Institute of Planning and Evaluation for Technology, Agriculture, Forestry, and Fisheries (IPET) as a Golden Seed Project (project number 213008-05-3-SB830) and funded by the Ministry of Oceans and Fisheries (MOF), Republic of Korea, and by Chonnam National University (Grant number: 2017-2657).

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Authors and Affiliations

  1. Department of Biology Education, Chonnam National University and Khumho Research Institute, Gwangju, 61186, Republic of Korea

    Jiwoong Wi & Dong-Woog Choi

  2. Seaweed Research Center, National Institute of Fisheries Science, Mokpo, 58746, Republic of Korea

    Eun-Jeong Park

  3. Aquatic Plant Variety Center, National Institute of Fisheries Science, Mokpo, 58746, Republic of Korea

    Mi Sook Hwang

  4. Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 34141, Republic of Korea

    Won-Joong Jeong

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  1. Jiwoong Wi
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Correspondence to Dong-Woog Choi.

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Key Message

PyMPV17 was identified as a desiccation response gene in Pyropia yezoensis, a desiccation-tolerant marine red alga. PyMPV17 is the Pyropia homolog of MPV17 associated with mitochondrial DNA depletion syndromes in mice. PyMPV17 reduces reactive oxygen species production and enhances osmotic stress tolerance in the alga.

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Wi, J., Park, EJ., Hwang, M.S. et al. PyMPV17, the MPV17 Homolog of Pyropia yezoensis (Rhodophyta), Enhances Osmotic Stress Tolerance in Chlamydomonas. Plant Mol Biol Rep 38, 39–47 (2020). https://doi.org/10.1007/s11105-019-01172-3

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