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Overexpression of IrlVHA-c, A Vacuolar-Type H+-ATPase c Subunit Gene from Iris lactea, Enhances Salt Tolerance in Tobacco

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Abstract

Vacuolar-type H+-ATPase (V-ATPase), a multi-subunit endomembrane proton pump, plays an important role in plant growth and response to environmental stresses. In the present study, transgenic tobacco that overexpressed the V-ATPase c subunit gene from Iris lactea (IrlVHA-c) was used to determine the function of IrlVHA-c. Quantitative PCR analysis showed that IrlVHA-c expression was induced by salt stress in I. lactea roots and leaves. Subcellular localization of green fluorescent protein (GFP) as marker combined with FM4-64 staining showed that the IrlVHA-c-GFP was localized to the endosomal compartment in tobacco cells. Compared with the wild-type, the IrlVHA-c transgenic tobacco plants exhibited greater seed germination rates, root length, fresh weight, and higher relative water content (RWC) of leaves under salt stress. Furthermore, the IrlVHA-c transgenic tobacco leaves have lower stomatal densities and larger stomatal apertures than wild-type. Under salt stress, superoxide dismutase (SOD) activity in the transgenic tobacco was significantly enhanced. Moreover, the level of malondialdehyde (MDA) in the transgenic tobacco was significantly lower than that in wild-type plants under salt stress. Taken together, these results suggested that the IrlVHA-c plays an important role in salt tolerance in transgenic tobacco by influencing stomatal movement and physiological changes.

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Acknowledgments

This work was supported by the Natural Science Foundation of Heilongjiang Province of China (C201112).

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Authors and Affiliations

  1. College of Horticulture, Northeast Agricultural University, Harbin, 150030, China

    Jingang Wang, Aimin Zhou, Yang Li, Shanshan Li, Xiaohui Zhang & Daidi Che

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  1. Jingang Wang
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  2. Aimin Zhou
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  3. Yang Li
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  4. Shanshan Li
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  5. Xiaohui Zhang
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  6. Daidi Che
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Correspondence to Daidi Che.

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Jingang Wang and Aimin Zhou are co-first authors.

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Wang, J., Zhou, A., Li, Y. et al. Overexpression of IrlVHA-c, A Vacuolar-Type H+-ATPase c Subunit Gene from Iris lactea, Enhances Salt Tolerance in Tobacco. Plant Mol Biol Rep 34, 877–885 (2016). https://doi.org/10.1007/s11105-015-0969-4

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