Abstract
Aims
Soil metabolites have a great influence on regulating the growth and development of plants and microbes in forest ecosystems. This study aims to reveal the characteristic metabolite profiles and their enriched metabolic pathways in the rhizosphere soil of Masson pine plantations at three different ages.
Method
The rhizosphere soil of Masson pine from 10, 31, and 52 years plantation was collected. The metabolites were analyzed using a soil pseudotargeted metabolomics approach and then the relationships between soil metabolites and chemical properties, enzyme activities were established.
Results
A total of 172 rhizosphere soil metabolites in 26 classes were identified. There was a decreasing trend in the total concentration of rhizosphere soil metabolites with increasing stand age. Seventy-two characteristic metabolites were screened, mainly saccharides, fatty acids, amino acids, phenolic acids, polyols and terpenoids. Among them, benzoic acid and galactonic acid contributed the most to the young forest, and stearic acid had the greatest influence on the near-mature forest plantation. Erythritol had great effects on the mature forest plantation. Seven metabolic pathways changed with increasing stand age. Correlation analysis showed that available potassium (AK) and soil alkaline protease (S-ALPT) were significantly positively correlated with phenolic acids, polyhydroxy carboxylic acids, etc.
Conclusion
The rhizosphere soil of Masson pine had different metabolite profiles at different developmental stages, and the soil nutrient pools were effectively improved. These results provide a reference for plantation management in Masson pine and a deeper understanding of forest metabolic characteristics.
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Data availability
The datasets used during the current study are available from the corresponding author on reasonable request.
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Funding
This work was supported by [the National Natural Science Foundation of China] (31960301), [the Postdoctoral Research Foundation of China] (2020M673583XB), [the Science and Technology Planning Project of Guizhou Province] (QKHPTRC[2018]5261 and QKHPTRC[2019]5102), [the Science and Technology Project of Guizhou Provincial Branch of the CNTC] (2023XM16) and [the Key Program for Science and Technology of CNTC] (110202202030), [the Young Elite Scientists Sponsorship Program of CNTC].
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Shuyue Qin: Methodology, Conceptualization, Data curation, Investigation, Formal analysis, Writing-Original draft preparation; Weichang Gao: Visualization, Validation; Yuan Jing: Investigation, Data curation; Wenxuan Quan: Investigation, Reviewing & Editing, Funding acquisition; Kai Cai: Writing, Conceptualization, Writing Reviewing, Supervision, Funding acquisition. All authors read and approved the final manuscript.
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Qin, S., Gao, W., Jing, Y. et al. Soil pseudotargeted metabolomics reveals that planting years of masson pine (Pinus massoniana) affect soil metabolite profiles and metabolic pathways. Plant Soil 496, 505–520 (2024). https://doi.org/10.1007/s11104-023-06378-9
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DOI: https://doi.org/10.1007/s11104-023-06378-9