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Phosphorus-acquisition characteristics and rhizosphere properties of wild barley in relation to genotypic differences as dependent on soil phosphorus availability

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Abstract

Aim

This study focused on P acquisition mechanisms of Hordeum spontaneum from P-impoverished soil by measuring plant growth, P acquisition and rhizosphere properties.

Methods

Two P-efficient genotypes (IS-22-30 and IS-22-25) and a P-inefficient genotype (IS-07-07) were subjected to a P supply of 0, 30 mg kg−1 (deficient P) and 60 mg kg−1 (sufficient P) in a rhizobag experiment. After 60 days, biomass, P concentration, and P uptake were analyzed. Changes in pH, P fractions and acid phosphatase activity in soil at different distances from the roots were determined, using a slicing technique.

Results

P-efficient genotypes showed greater biomass and plant P content, but lower plant P concentration than the P-inefficient genotype. The average seed P content was 113 and 176 μg P per seed of P-efficient genotypes and P-inefficient genotype, respectively. P-efficient genotypes showed P uptake of 3.0 and 7.9 mg pot−1 at 0 and 30 mg P kg−1; while that of P-inefficient genotype was 1.0 and 5.3 mg pot−1, respectively. Acid phosphatase activity was significantly enhanced in the soil-root interface and P-efficient genotypes demonstrated 54% greater activity of rhizosphere acid phosphatase than the P-inefficient genotype. Rhizosphere P fractions showed that P utilized by the two genotypes mainly came from H2O-Pi, NaHCO3-Pi, and NaOH-Pi, while the P-efficient genotypes markedly depleted NaHCO3-Po, NaOH-Po and HCl-Pi, and the depletion zone extended to 4 mm under P deficiency.

Conclusions

These results indicate the potential to sustain more growth and greater P acquisition by P-efficient genotypes of wild barley from P-deficient soil through changes of rhizosphere P fractions and enhancing rhizosphere acid phosphatase activity.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (31401377), the Sichuan Science and Technology Support Project (2013NZ0029, 2013NZ0044) and the Key Project of Education Department in Sichuan Province (14ZA0002). The authors wish to thank the Triticeae Research Institute for providing seeds of wild barley and also appreciate professor Hans Lambers (University of Western Australia) for his useful comments regarding the contents and language of this manuscript.

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Authors and Affiliations

  1. College of Resources, Sichuan Agricultural University, 211 Huimin Road, Chengdu, Sichuan, 611130, China

    Daihua Ye, Xizhou Zhang, Tingxuan Li & Guangdeng Chen

  2. Soil and Fertilizer Station, Leshan Agricultural Bureau, 222 Baiyang Eest Road, Leshan, Sichuan, 614001, China

    Jing Xu

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  1. Daihua Ye
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  2. Xizhou Zhang
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  3. Tingxuan Li
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  4. Jing Xu
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Correspondence to Xizhou Zhang or Tingxuan Li.

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Responsible Editor: John Hammond.

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Ye, D., Zhang, X., Li, T. et al. Phosphorus-acquisition characteristics and rhizosphere properties of wild barley in relation to genotypic differences as dependent on soil phosphorus availability. Plant Soil 423, 503–516 (2018). https://doi.org/10.1007/s11104-017-3530-4

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