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Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties

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Abstract

Aims

The study aimed to assess comparatively the accuracy and efficiency of three culture media protocols for estimating black-foot disease pathogens populations in soils and to examine how shifts in the abundance and composition of black-foot pathogens correspond to changes in specific soil properties.

Methods

Firstly, culture media were compared by evaluating the mycelial growth of selected black-foot pathogens and by estimating the population of Dactylonectria torresensis from artificially infested soils. Secondly, the most efficient culture medium was selected for estimating the viable propagules of black-foot disease pathogens in eight naturally infested soils. An analysis of the soil physicochemical properties was conducted. Data were statistically analyzed in order to explore possible relationships between the studied variables.

Results

Glucose-Faba Bean Rose Bengal Agar (GFBRBA) was selected as the most efficient culture medium. All naturally infested soils tested positive for the presence of black-foot pathogens. D. torresensis was the most frequently isolated species, followed by Dactylonectria alcacerensis and Ilyonectria liriodendri. A positive relationship between calcium carbonate and the Colony-Forming Units (CFUs) level of black-foot pathogens in soil was obtained.

Conclusions

In this study, we provide an early, specific, and accurate detection of viable propagules of black-foot pathogens in soil, which is critical to understand the ecology of these fungi and to design effective management strategies.

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Abbreviations

CEC:

Cation Exchange Capacity

CFU:

Colony-Forming Unit

EC:

Electric conductivity

GFBA:

Glucose-Faba Bean Agar

GFBRGA:

Glucose-Faba Bean Rose Bengal Agar

ITS:

Internal Transcribed Spacer

MRBA:

Modified Rose Bengal Agar

NMDS:

Non-Metric Multidimensional Scaling Analysis

LSD:

Least Significant Difference

PCA:

Principal Component Analysis

PCR:

Polymerase Chain Reaction

PDA:

Potato Dextrose Agar

PDAC:

Potato Dextrose Agar supplemented with 250 mg l−1 of chloramphenicol

SNA:

Spezieller Nährstoffarmer Agar

SOM:

Soil Organic Matter

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Acknowledgements

The research was funded by CAR (Government of La Rioja, Spain), under the project “Characterization, epidemiology and control of fungal trunk pathogens of grapevine in La Rioja” (project number R-03-16). We thank “Instituto Navarro de Tecnologías e Infraestructuras Agroalimentarias” (INTIA), Spain, which collected the soil samples from the field in Olite for further use in the experiment. We thank P. Yécora and M. Andrés for technical assistance. David Gramaje was supported by the DOC-INIA program from the National Institute for Agronomic Research (INIA), co-funded by the European Social Fund. Carmen Berlanas was supported by the FPI-INIA program from the INIA. Beatriz López was supported by the PhD program from the Government of La Rioja.

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  1. Instituto de Ciencias de la Vid y del Vino (ICVV), Consejo Superior de Investigaciones Científicas - Universidad de la Rioja - Gobierno de La Rioja, Ctra. LO-20 Salida 13, Finca La Grajera, 26071, Logroño, Spain

    Carmen Berlanas, Beatriz López-Manzanares & David Gramaje

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  1. Carmen Berlanas
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  2. Beatriz López-Manzanares
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Correspondence to David Gramaje.

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Berlanas, C., López-Manzanares, B. & Gramaje, D. Estimation of viable propagules of black-foot disease pathogens in grapevine cultivated soils and their relation to production systems and soil properties. Plant Soil 417, 467–479 (2017). https://doi.org/10.1007/s11104-017-3272-3

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