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Inoculation of Fe/Mn-oxidizing bacteria enhances Fe/Mn plaque formation and reduces Cd and As accumulation in Rice Plant tissues

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Abstract

Aims

Iron plaques can prevent the uptake of heavy metals on the root surface of wetland plants. The Fe and Mn oxides produced by microorganisms are major Fe/Mn-oxidizing agents in the environment. This study is the first to investigate the influence of Fe/Mn-oxidizing bacteria on Fe/Mn plaque formation and the uptake of Cd and As on rice tissues.

Methods

The oxide indicators Fe (1,10-phenanthrolin) and Mn (leucoberbelin blue) were used to determine the Fe/Mn oxidation ratios and Fe(II)/Mn(II) oxidation ability of the isolates. Fe/Mn plaque formation on the root was determined by the dithionite–citrate–bicarbonate method. The Cd and As concentrations in different organs of rice were determined by ICP-MS.

Results

Two bacterial strains D54 and TWD-2 with Fe(II) and Mn(II) oxidation ability were isolated from heavy metal-contaminated paddy soils. Fe/Mn plaque formation on rice roots was strongly enhanced via inoculation of Fe/Mn-oxidizing strains. The inoculation of strains D54 and TWD-2 and the addition of Fe(II) significantly decreased the Cd/As concentration in the root, leaf, glume and brown rice grain of the rice plant. Moreover, the photosynthetic rate of rice leaf was enhanced by inoculation of the strains D54 and TWD-2.

Conclusions

The formation of Fe/Mn plaques on rice roots can be stimulated via the inoculation of the Fe/Mn-oxidizing strains D54 and TWD-2 combined with the addition of exogenous Fe(II). Increased Fe/Mn plaque formation promotes the deposition of metals to the root surface, thereby limiting the uptake and transfer of Cd/As in rice tissues.

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Acknowledgments

This research was financially supported by the Central Public Research Institutes Basic Funds for Research and Development (Agro-Environmental Protection Institute, Ministry of Agriculture) and the National Natural Science Foundation of China (Nos. 41473115 and 41001191).

Author information

Authors and Affiliations

  1. Department of Agriculture, Guangxi University, Nanning, 530003, People’s Republic of China

    Ming Fang Dong & Zhi Lian Fan

  2. Innovation Team of Remediation for Heavy Metal Contaminated Farmland of Chinese Academic of Agricultural Sciences, Institute of Agro-Environmental Protection, Ministry of Agriculture, Tianjin, 300191, People’s Republic of China

    Ming Fang Dong, Ren Wei Feng, Rui Gang Wang, Yang Sun, Ying Ming Xu & Jun Kang Guo

  3. Agro-Environmental Protection Institute, Ministry of Agriculture, Tianjin, 300191, People’s Republic of China

    Yong Zhen Ding

Authors
  1. Ming Fang Dong
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  2. Ren Wei Feng
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  3. Rui Gang Wang
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  4. Yang Sun
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  5. Yong Zhen Ding
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  6. Ying Ming Xu
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  7. Zhi Lian Fan
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  8. Jun Kang Guo
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Correspondence to Zhi Lian Fan or Jun Kang Guo.

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Responsible Editor: Henk Schat.

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Dong, M.F., Feng, R.W., Wang, R.G. et al. Inoculation of Fe/Mn-oxidizing bacteria enhances Fe/Mn plaque formation and reduces Cd and As accumulation in Rice Plant tissues. Plant Soil 404, 75–83 (2016). https://doi.org/10.1007/s11104-016-2829-x

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  • DOI: https://doi.org/10.1007/s11104-016-2829-x

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