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Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance

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Abstract

Powdery mildew species Oidium neolycopersici (On) can cause serious yield losses in tomato production worldwide. Besides on tomato, On is able to grow and reproduce on Arabidopsis. In this study we screened a collection of activation-tagged Arabidopsis mutants and identified one mutant, 3221, which displayed resistance to On, and in addition showed a reduced stature and serrated leaves. Additional disease tests demonstrated that the 3221 mutant exhibited resistance to downy mildew (Hyaloperonospora arabidopsidis) and green peach aphid (Myzus persicae), but retained susceptibility to bacterial pathogen Pseudomonas syringae pv tomato DC3000. The resistance trait and morphological alteration were mutually linked in 3221. Identification of the activation tag insertion site and microarray analysis revealed that ATHB13, a homeodomain-leucine zipper (HD-Zip) transcription factor, was constitutively overexpressed in 3221. Silencing of ATHB13 in 3221 resulted in the loss of both the morphological alteration and resistance, whereas overexpression of the cloned ATHB13 in Col-0 and Col-eds1-2 backgrounds resulted in morphological alteration and resistance. Microarray analysis further revealed that overexpression of ATHB13 influenced the expression of a large number of genes. Previously, it was reported that ATHB13-overexpressing lines conferred tolerance to abiotic stress. Together with our results, it appears that ATHB13 is involved in the crosstalk between abiotic and biotic stress resistance pathways.

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Acknowledgments

This work was funded by the Technological Top Institute Green Genetics, the Netherlands (TTI-GG:2CC038RP) together with Keygene N.V, Syngenta and Rijk Zwaan Breeding B.V.

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  1. Robin P. Huibers

    Present address: Enza Zaden Beheer B.V., Haling 1E, 1602 DB, Enkhuizen, The Netherlands

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  1. Wageningen UR Plant Breeding, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Dongli Gao, Michela Appiano, Robin P. Huibers, Xi Chen, Annelies E. H. M. Loonen, Richard G. F. Visser, Anne -Marie A. Wolters & Yuling Bai

  2. Graduate School Experimental Plant Sciences, Wageningen University and Research Centre, Droevendaalsesteeg 1, 6708 PB, Wageningen, The Netherlands

    Dongli Gao, Richard G. F. Visser, Anne -Marie A. Wolters & Yuling Bai

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Dongli Gao, Michela Appiano and Robin P. Huibers contributed equally.

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Gao, D., Appiano, M., Huibers, R.P. et al. Activation tagging of ATHB13 in Arabidopsis thaliana confers broad-spectrum disease resistance. Plant Mol Biol 86, 641–653 (2014). https://doi.org/10.1007/s11103-014-0253-2

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