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Common tools for pituitary adenomas research: cell lines and primary cells

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Abstract

Purpose

Pituitary tumor is the common primary brain tumor in humans. For further studying the pathogenesis and new therapeutic targets of pituitary adenoma, cell lines and primary cells are necessary tools. Different from primary cells that have short survival time and hormone secretion maintenance time, cell lines would be endowed with immortal characteristics under the help of gene modification. This review is to explore whether these cell lines still have similar pathophysiological changes in pituitary adenoma cells and methods to prolong the lifespan of pituitary adenoma primary cells.

Results

In the cell lines summarized in the review, HP75, PDFS, HPA and GX were derived from human pituitary adenomas. It was found that the cell lines commonly used in articles published between January 2014 and July 2019 were GH3, AtT20, MMQ, GH4C1, HP75 and TtT/GF. Besides, it was glad that many methods had been used to prolong the lifespan and maintain characteristics of pituitary adenoma primary cells.

Conclusion

The paper reviews most of pituitary adenoma cell lines that have been successfully established since 1968 and the relevant situation of primary culture of pituitary adenoma cells. Obviously, it requires us to make more efforts to obtain human pituitary adenoma cell lines and prolong the lifespan of pituitary adenoma primary cells with maintaining their morphology and ability to secret hormones.

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Funding

This study was funded by Research Project of Traditional Chinese Medicine Bureau of Guangdong Province (Grant No. 20173003).

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Authors and Affiliations

  1. Department of Histology and Embryology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, Guangdong, China

    Ziyan Zhu, Weiwei Cui & Yonghong Zhu

  2. Department of Neurosurgery and Pituitary Tumor Center, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou, Guangdong, China

    Dimin Zhu & Nailin Gao

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  1. Ziyan Zhu
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  2. Weiwei Cui
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  3. Dimin Zhu
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Contributions

ZZ and WC have contributed equally to this work. ZZ and WC drafted the manuscript and performed the literature search. DZ and NG performed the literature search. YZ had the idea for the article and critically revised the work.

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Correspondence to Yonghong Zhu.

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Zhu, Z., Cui, W., Zhu, D. et al. Common tools for pituitary adenomas research: cell lines and primary cells. Pituitary 23, 182–188 (2020). https://doi.org/10.1007/s11102-019-01003-4

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