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The Controlled Release and Anti-Inflammatory Activity of a Tetramethylpyrazine-Loaded Thermosensitive Poloxamer Hydrogel

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Abstract

Purpose

Tetramethylpyrazine-loaded poloxamer hydrogel materials were studied to achieve the controlled release of tetramethylpyrazine.

Methods

First, hydrogels having different concentrations of poloxamer 407 and poloxamer 188 were prepared. The gelling temperature and viscosity were measured. Second, we investigated the tetramethylpyrazine release rate from the thermosensitive poloxamer hydrogel materials in vitro and ex vivo. Finally, further study of the pharmacological efficacy of the tetramethylpyrazine-loaded thermosensitive poloxamer hydrogel materials was also investigated in vivo.

Results

The in vitro, ex vivo and in vivo experimental results showed that the tetramethylpyrazine-loaded poloxamer hydrogel with the appropriate gelling temperature, good adhesion and easy preparation controlled the release of tetramethylpyrazine.

Conclusions

The hydrogel with the suitable nasal temperature and a satisfactory adhesion was selected. The relevant tests were carried out, including the determination of the concentration of drugs in the brain homogenate and the anti-inflammatory test after different modes of administration. So the poloxamer hydrogel was a novel carrier to deliver TMP to pass across the blood brain barrier via nasal administration.

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ACKNOWLEDGMENTS AND DISCLOSURES

This project was financially supported by the Natural Science Foundation of Anhui Province of China (1608085MH227), the 2017 Anhui University of Chinese Medicine annual innovation training program for College Students (2017171, 2017142), the Natural Science Fund of Anhui University of Chinese Medicine (2010zr004A), the Kangyuan Fund (KYCX201001) and the Anhui province science and technology special fund project (13Z04013), the Natural Science Foundation (61573615). The authors report no declarations of interest. The authors alone are responsible for the content.

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Author notes

  1. Hongmei Xia and Hongliu Jin contributed equally to this work.

Authors and Affiliations

  1. College of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230038, People’s Republic of China

    Hongmei Xia, Hongliu Jin & Zhiqing Cheng

  2. School of Life Science, University of Science and Technology of China, Hefei, 230027, People’s Republic of China

    Yongfeng Cheng

  3. Zhaoke (Hefei) Pharmaceutical Co. Ltd., Hefei, 230088, People’s Republic of China

    Yinxiang Xu

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  1. Hongmei Xia
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Xia, H., Jin, H., Cheng, Y. et al. The Controlled Release and Anti-Inflammatory Activity of a Tetramethylpyrazine-Loaded Thermosensitive Poloxamer Hydrogel. Pharm Res 36, 52 (2019). https://doi.org/10.1007/s11095-019-2580-0

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