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Fundamentals of Pharmacokinetics to Assess the Correlation Between Plasma Drug Concentrations and Different Blood Sampling Methods

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Abstract

Purpose

Various blood collection methods were developed and used in the pharmacokinetic evaluation of drugs. However, the influence of different blood sampling methods on plasma drug concentrations has not been clarified. In the present study, we aimed to determine whether the plasma concentration of a target drug changes based on the collection site and elucidate the mechanism responsible for this change.

Methods

We compared three blood sampling methods commonly used in small animals. Eight clinical drugs were selected and administered to rats simultaneously via intracardiac injection or oral gavage. Blood samples were collected from different sites at the same individual, and pharmacokinetic properties of the drugs were then evaluated.

Results

Study results showed that the maximum plasma concentration or area under the curve of three study drugs was significantly higher in rats when blood was sampled from the carotid artery than when it was sampled from the caudal vein or by tail snip.

Conclusions

Pharmacokinetics of certain drugs may differ based on the blood sampling site. The acid-base properties of drugs may influence pharmacokinetic evaluation. The rate and extent of drug distribution may also cause such differences and have significant effects on plasma drug levels.

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Authors and Affiliations

  1. Graduate Institute of Pharmacy, National Defense Medical Center, Taipei, Taiwan, Republic of China

    Wei-Ching Chen, Ying-Ning Shih & Hong-Jaan Wang

  2. School of Pharmacy, National Defense Medical Center, R9304, No.161, Sec. 6, Min-Chuan E. Rd., Neihu District, Taipei 114, Taiwan, Republic of China

    Pei-Wei Huang, Wan-Ling Yang & Hong-Jaan Wang

  3. Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan, Republic of China

    Yen-Lun Chen

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  1. Wei-Ching Chen
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  2. Pei-Wei Huang
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Correspondence to Hong-Jaan Wang.

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Chen, WC., Huang, PW., Yang, WL. et al. Fundamentals of Pharmacokinetics to Assess the Correlation Between Plasma Drug Concentrations and Different Blood Sampling Methods. Pharm Res 36, 32 (2019). https://doi.org/10.1007/s11095-018-2550-y

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  • DOI: https://doi.org/10.1007/s11095-018-2550-y

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