ABSTRACT
Purpose
In general, the surface functionalization of polymeric nanoparticles is carried out by covalently bounding ligands to the nanoparticle surface. This process can cause a lack or decrease of the ligand specificity to its target receptor, besides the need of purification steps. We proposed a ligand-metal-chitosan-lecithin complex as a new strategy to functionalize the surface of biodegradable nanoparticles.
Methods
One pot synthesis of scFv anti-LDL(−)-functionalized nanocapsules was carried out by self-assembly and interfacial reactions. Particle sizing techniques, lipid peroxidation and molecular recognition by enzyme linked immuno sorbent assays were carried out.
Results
The selected formulation had unimodal size distribution with mean diameter of about 130 nm. The metals in the complex did not enhance the oxidative stress, and the scFv anti-LDL(−)-functionalized nanocapsules recognized LDL(−) and did not react with native LDL indicating the maintenance of the active site of the fragment.
Conclusions
The one pot synthesis, using the ligand-metal-chitosan-lecithin complex to functionalize the surface of the biodegradable nanocapsules, maintained the active site of the antibody fragment making the device interesting for applications in nanomedicine.
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Abbreviations
- DLS:
-
Dynamic light scattering
- ELISA:
-
Enzyme linked immuno sorbent assay
- LD:
-
Laser diffractometry
- LDE:
-
Laser Doppler electrophoresis
- LDL:
-
Low density lipoprotein
- LDL(−):
-
Electronegative low density lipoprotein
- LNC:
-
Lipid-core nanocapsules
- MCMN:
-
Metal complex multi-wall nanocapsules
- MDA:
-
Malondialdehyde
- MN:
-
Multi-wall nanocapsules
- nLDL:
-
Native low density lipoprotein
- NTA:
-
Nanoparticle tracking analysis
- PCL:
-
Poly(ε-caprolactone)
- PDI:
-
Polydispersity index
- Phe:
-
Phenylalanine
- scFv anti-LDL(−):
-
Anti-electronegative LDL single-chain fragment variable
- SPAN:
-
Polydispersity
- TBA:
-
Thiobarbituric acid
- TBARS:
-
Thiobarbituric acid reactive substances
- TEM:
-
Transmission electron microscopy
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ACKNOWLEDGMENTS AND DISCLOSURES
EAB thanks CAPES/Brazil for his fellowship. The authors thank PRONEX e PRONEM FAPERGS/CNPq, FAPESP, INCT-IF/CNPq, CNPq/Brasilia/Brazil, Universal CNPq/MCTI and Rede Nanotecnologia Farmaceutica CAPES (Brazil) for grant financial supports.
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Bender, E.A., Cavalcante, M.F., Adorne, M.D. et al. New strategy to surface functionalization of polymeric nanoparticles: one-pot synthesis of scFv anti-LDL(−)-functionalized nanocapsules. Pharm Res 31, 2975–2987 (2014). https://doi.org/10.1007/s11095-014-1392-5
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DOI: https://doi.org/10.1007/s11095-014-1392-5