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Application of Impedance Technology to the Determination of Microbial Contamination of Medicinal Plant Raw Materials

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Pharmaceutical Chemistry Journal Aims and scope

An impedance technology or conductometric method in microbiological control is an indirect culture method for the determination of microorganisms using measurements of the impedance, i.e., electrical resistance to an alternating current in a growth medium as its chemical composition is transformed by the growth and metabolic activity of microorganisms. Traditional pharmacopoeial methods (double-layer agar method, surface inoculation, submerged cultivation) are used in practice for analysis. The conductometric method and the ATP-luminescent method for determining the total number of microorganisms can be used as alternative methods that significantly reduce the time of analysis and the amounts of consumables. The possibility of using impedance technology (conductometry method) for the determination of the total number of aerobic microorganisms in samples of medicinal plant raw materials with high levels of microbial contamination was examined by us. The aim of the study was to determine the total number of aerobic microorganisms in 1 g (mL) of herbal medicinal raw materials by impedance conductometric and pharmacopoeial cup agar methods. The results from determining the number of aerobic microorganisms using the cup pharmacopoeial and conductometric methods agreed with a correlation coefficient of 0.964.

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Authors and Affiliations

  1. St. Petersburg Chemical and Pharmaceutical University, 14A Prof. Popova St., St. Petersburg, 197022, Russia

    E. P. Anan’eva, O. Yu. Bogdanova, S. V. Gurina & V. S. Sibirtsev

Authors
  1. E. P. Anan’eva
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  2. O. Yu. Bogdanova
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  3. S. V. Gurina
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  4. V. S. Sibirtsev
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Corresponding author

Correspondence to O. Yu. Bogdanova.

Additional information

Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 57, No. 6, pp. 59 – 64, June, 2023.

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Anan’eva, E.P., Bogdanova, O.Y., Gurina, S.V. et al. Application of Impedance Technology to the Determination of Microbial Contamination of Medicinal Plant Raw Materials. Pharm Chem J 57, 913–917 (2023). https://doi.org/10.1007/s11094-023-02967-1

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  • DOI: https://doi.org/10.1007/s11094-023-02967-1

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