A method for simultaneous quantitative analysis of dihydroquercetin and secoisolariciresinol using HPLC with a diode-array detector (DAD) was developed, validated, and used for quantitative determination of the main polyphenol components of larch (Larix dahurica Turcz.) knotwood. The content of flavonoid dihydroquercetin was 59.89 ± 0.8%; of lignan secoisolariciresinol, 22.38 ± 0.24%. The analytical range of the method was 50 – 1500 μg/mL for dihydroquercetin and 100 – 1500 μg/mL for secoisolariciresinol. The agreement of the results (RSD, %) was ≤1.2%.
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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 57, No. 5, pp. 55 – 59, May, 2023.
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Voronin, K.S., Fenin, A.A., Zhevlakova, A.K. et al. Development and Validation of a Method for Simultaneous Quantification of Dihydroquercetin and Secoisolariciresinol. Pharm Chem J 57, 740–744 (2023). https://doi.org/10.1007/s11094-023-02948-4
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DOI: https://doi.org/10.1007/s11094-023-02948-4