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Method for Obtaining and Hypolipidemic Activity Assay of a Lipolytic Enzyme from Nigella sativa Seeds

  • DRUG SYNTHESIS METHODS AND MANUFACTURING TECHNOLOGY
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Pharmaceutical Chemistry Journal Aims and scope

A lipolytic enzyme was isolated from seeds of Nigella sativa after pressing the oil to develop a waste-free technology. An enzyme with a maximum lipolytic activity of 150,000 U/g was obtained by selecting the optimum temperature (37°C) and pH (10.5). A comparative study of the lipolytic enzyme and sebelipase alfa in the Tween hyperlipidemia model in rats showed that the former decreased the concentration of total cholesterol by 19.6%, which indicated the expediency of using this enzyme to improve absorption processes in the gastrointestinal tract.

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Authors and Affiliations

  1. A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, 83 Mirzo Ulugbeka St., Tashkent, Uzbekistan, 100125

    T. A. Khudoiberdiev & Yu. I. Oshchepkova

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  1. T. A. Khudoiberdiev
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  2. Yu. I. Oshchepkova
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Correspondence to Yu. I. Oshchepkova.

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Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 56, No. 6, pp. 44 – 47, June, 2022.

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Khudoiberdiev, T.A., Oshchepkova, Y.I. Method for Obtaining and Hypolipidemic Activity Assay of a Lipolytic Enzyme from Nigella sativa Seeds. Pharm Chem J 56, 862–865 (2022). https://doi.org/10.1007/s11094-022-02719-7

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  • DOI: https://doi.org/10.1007/s11094-022-02719-7

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