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Comparative Study of the Efficacy of Different Artemisia Cina Extracts and their Nanoparticulated Forms against A549 Lung Cancer Cell Line

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Pharmaceutical Chemistry Journal Aims and scope

The present study aimed to investigate the possible antiproliferative effects of the plant extracts of Artemisia cina on lung cancer, and whether the nanoencapsulation of the plant extracts in the form of nanoemulsion would potentiate their therapeutic efficacy. Soxhlet extraction of the freshly collected shade-dried plant using n-hexane and methanol was performed, and the two extracts were loaded within nanoemulsion using the water dilution method. The nanoemulsion was characterized for its particle size, zeta potential, polydispersity, physicochemical stability under refrigeration conditions, and was morphologically visualized using transmission electron microscopy. The antiproliferative activity of the extracts and their nanoforms was tested in A549 lung cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Results revealed the successful preparation of the plant extract-loaded nanoemulsions with suitable particle size from 15-16 nm, a homogenous dispersion with polydispersity index (PDI) ranging from 0.23-0.31, and neutral surface charge. The nanoemulsions displayed insignificant changes in their physicochemical properties after storage for 3 months, and their spherical morphology was confirmed using transmission electron microscopy. Both n-hexane and methanol extracts di splayed antiproliferative activity against A549 cells, with IC50 values of 35.96 ± 1.7 and 41.6 ± 2.8 μg/ml, respectively. On the other hand, their respective nanoparticulated forms displayed superior antiproliferative activity, with IC50 values of 12.59 ± 0.7 and 5.6 ± 0.4 μg/ml, respectively. Therefore, it can be concluded that nanoencapsulation of plant extracts would significantly potentiate their antiproliferative activity, which paves the way towards a more effective anticancer therapy.

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References

  1. R. S. Herbst, J. V. Heymach, and S. M. Lippman, New Eng. J. Med., 359, 1367 – 1380 (2008).

    Article  CAS  Google Scholar 

  2. R. D. Neal, F. Sun, J. D. Emery, and M. E. Callister, BMJ, 365, 11725 (2019).

    Google Scholar 

  3. J. Ferlay, E. Steliarova-Foucher, J. Lortet-Tieulent, et al., Eur. J. Cancer, 49, 1374 – 1403 (2013).

    Article  CAS  Google Scholar 

  4. American Cancer Society: Key Statistics for Lung Cancer 2019, American Cancer Society. Atlanta, USA. January, 2019. https: //www.cancer.org/cancer/non-small-cell-lung-cancer/about/keystatistics.html

  5. P. Goldstraw, K. Chansky, J. Crowley, et al., J. Thorac. Oncol., 11, 39 – 51 (2016).

    Article  Google Scholar 

  6. K. S. Bora and A. Sharma, Pharm. Biol., 49, 101 – 109 (2011).

    Article  Google Scholar 

  7. R. N. Chopra, S. L. Nayar, and I. C. Chopra, CSIR New Delhi, 26, 2 (1956).

    Google Scholar 

  8. A. Narwaria, R. L. Khosa, and S. K. Dhar, Anc. Sci. Life, 14, 10 – 15 (1994).

    PubMed  PubMed Central  CAS  Google Scholar 

  9. T. Efferth. Biochem. Pharmacol., 139, 56 – 70 (2017).

    Article  CAS  Google Scholar 

  10. G. Lian, F. Li, Y. Yin, L. Chen, and J. Yang, JBUON, 23, 73 – 78 (2018).

    PubMed  Google Scholar 

  11. T. Rasheed, M. Bilal, H. M. N. Iqbal, and C. Li, Colloids Surf. B: Biointerfaces, 158, 408 – 415 (2017).

    Article  CAS  Google Scholar 

  12. B. Mousavi, F. Tafvizi, and S. Z. Bostanabad, Artif. Cells Nanomed. Biotechnol., 46, 499 – 510 (2018).

    Article  CAS  Google Scholar 

  13. S. Aldalaen, R. I. El-Gogary, and M. Nasr, Drug Dev. Ind. Pharm., 45, 55 – 62 (2019).

    Article  CAS  Google Scholar 

  14. M. Fadel, K. Kassab, D. A. Abd El Fadeel, et al., Drug Dev. Ind. Pharm., 44, 1809 – 1816 (2018).

    Article  CAS  Google Scholar 

  15. R. Said-Elbahr, M. Nasr, M. A. Alhnan, et al., Eur. J. Pharm. Biopharm., 103, 1 – 12 (2016).

    Article  CAS  Google Scholar 

  16. L. Ramzy, M. Nasr, A. A. Metwally, and G. A. S. Awad, Eur. J. Pharm. Sci., 104, 273 – 292 (2017).

    Article  CAS  Google Scholar 

  17. M. Nasr, S. Abdel-Hamid, N. H. Moftah, et al., Curr. Drug Deliv., 14, 426 – 432 (2017).

    Article  CAS  Google Scholar 

  18. M. Nasr, and S. Abdel-Hamid, Drug Dev. Ind. Pharm., 42, 636 – 643 (2016).

    Article  CAS  Google Scholar 

  19. O. Ashraf, M. Nasr, M. Nebsen, et al., Int. J. Pharm., 539, 83 – 94 (2018).

    Article  CAS  Google Scholar 

  20. M. Nasr, S. Mansour, N. D. Mortada, and A. A. Elshamy, J. Microencapsul., 25, 499 – 512 (2008).

    Article  CAS  Google Scholar 

  21. M. Nasr, S. Mansour, N. D. Mortada, and A. A. El Shamy, AAPS PharmSciTech, 9, 154 – 162 (2008).

    Article  CAS  Google Scholar 

  22. M. A. Mouez, M. Nasr, M. Abdel-Mottaleb, et al., Int. J. Biol. Macromol., 93, 591 – 599 (2016).

    Article  CAS  Google Scholar 

  23. R. I. El-Gogary, S. A. A. Gaber, and M. Nasr. Sci. Rep., 9, 11064 (2019).

    Article  CAS  Google Scholar 

  24. L. Deng, F. Que, H. Wei, et al., PLoS One, 10, e0127291 (2015).

  25. D. Obistioiu, R. T. Cristina, I. Schmerold, et al., Chem. Cent. J., 8, 1 – 11 (2014).

    Article  CAS  Google Scholar 

  26. G. Tajadod, A. Mazooji, F. Salimpour, et al., Ann. Biol. Res., 3, 385 – 389 (2012).

    CAS  Google Scholar 

  27. J. J. Mills, R. S. Chari, J. J. Boyer, et al., Cancer Res., 55, 979 – 983 (1995).

    PubMed  CAS  Google Scholar 

  28. X. Song, X. Wen, J. He, et al., J. Funct. Foods, 52, 648 – 662 (2019).

    Article  CAS  Google Scholar 

  29. A. Taleghani, S. A. Emami, and Z. Tayarani-Najaran, Bioorg. Med. Chem., 28, 1 – 22 (2020).

    Article  CAS  Google Scholar 

  30. B. Koul, P. Taak, A. Kumar, et al., J. Glyco. Lipid, 7 (2017).

  31. J. Y. Lee, E. J. Chang, H. J. Kim, et al., Arch. Pharm. Res., 25, 313 – 319 (2002).

    Article  CAS  Google Scholar 

  32. J. Suresh, K. Mruthunjaya, N. Paramakrishnan, and M. N. Naganandhini, Int. J. Curr. Chem. Res., 3, 49 – 52 (2011).

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Faculty of Pharmacy, Mu’tah University, Al-Karak, 61710, Jordan

    Aseel Khaled Al Sarayrah & Rasha Zakaria Al Tarawneh

  2. Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street 1, Cairo, 11566, Egypt

    Maha Nasr

  3. Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Organization of African Unity Street 1, Cairo, 11566, Egypt

    Sherif S. Ebada

  4. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mu’tah University, Al-Karak, 61710, Jordan

    Sherif S. Ebada

Authors
  1. Aseel Khaled Al Sarayrah
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  2. Rasha Zakaria Al Tarawneh
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  3. Maha Nasr
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  4. Sherif S. Ebada
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Correspondence to Maha Nasr.

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Al Sarayrah, A.K., Al Tarawneh, R.Z., Nasr, M. et al. Comparative Study of the Efficacy of Different Artemisia Cina Extracts and their Nanoparticulated Forms against A549 Lung Cancer Cell Line. Pharm Chem J 54, 938–942 (2020). https://doi.org/10.1007/s11094-020-02300-0

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  • DOI: https://doi.org/10.1007/s11094-020-02300-0

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