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Design and Physicochemical Characterization of Lysozyme Loaded Niosomal Formulations as a New Controlled Delivery System

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Pharmaceutical Chemistry Journal Aims and scope

Lysozyme loaded niosomes containing various molar ratios of two kinds of surfactants were prepared and the properties of these niosomal formulations were studied. The results revealed that the size of niosomes varied between 240.06 ± 32.41 and 895.2 ± 20.84 nm. Formulations with the lowest size and no precipitation had entrapment efficiencies ranging from 60.644 ± 3.310 to 66.333 ± 1.98%. Their controlled release profiles after 48 h were 15.67, 20.67 and 31.50%. After 2 months, the most stable formulation in terms of size, PDI, zeta potential, and entrapment efficiency was used to study the secondary structures of lysozyme in niosomal and free forms. Lysozyme loaded niosome and lysozyme adsorbed on the surface of niosome fell into one category in terms of the formation of α-helix,β -sheet, and turn structures. This study suggests that niosomes could be a promising delivery system for lysozyme with prolonged release profiles, which can be used in pharmaceutical and food industries.

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Acknowledgements

This project was financially supported by a scholarship of education center of the Pasteur Institute of Iran to SS, PhD student.

Conflict of Interest

Authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Nano biotechnology, New Technology Research Group, Pasteur Institute of Iran, Tehran, Iran

    Somayeh Sadeghi, Reza Ahangari Cohan, Iman Akbarzadeh, Haleh Bakhshandeh & Dariush Norouzian

  2. Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran

    Somayeh Sadeghi & Parastoo Ehsani

  3. Department of Medical Biotechnology, Biotechnology Research Centre, Pasteur Institute of Iran, Tehran, Iran

    Soroush Sardari

  4. Department of Chemical and Petroleum Engineering, Biotechnology Research Centre, Sharif University of Technology, Tehran, Iran

    Iman Akbarzadeh

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  1. Somayeh Sadeghi
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Correspondence to Haleh Bakhshandeh.

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Sadeghi, S., Ehsani, P., Cohan, R.A. et al. Design and Physicochemical Characterization of Lysozyme Loaded Niosomal Formulations as a New Controlled Delivery System. Pharm Chem J 53, 921–930 (2020). https://doi.org/10.1007/s11094-020-02100-6

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  • DOI: https://doi.org/10.1007/s11094-020-02100-6

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