We present here a method for training rats to perform electrical self-stimulation in response to elevating the head using a telemetry device to record extracellular dopamine levels. Experiments reported by Olds were the first to show that the response to electrical stimulation of positive reinforcement zones in rats consists of natural exploratory behavior with the aim of seeking a source of reward. It has been suggested that a natural behavioral act, specifically raising the head, is able to accelerate the development and stabilization of the self-stimulation response. Changes in head position can serve as an internal proprioceptive conditioned signal. Experiments were carried out in an annular chamber, where the ventral tegmental area (VTA) was stimulated using a telemetry device when the rat’s head was raised to 38°. The self-stimulation response to headlifts developed and stabilized during the first day of training. For comparison, self-stimulation by pedal-pressing produced clear reproduction of responses only on training day 3 after the procedures of “pushing” the pedal on training day 1 and being “pushed off” the pedal on training day 2; stabilization of the response was observed only on training day 4. After stabilization of the self-stimulation response in rats, fast-scan cyclic voltammetry was used to record extracellular dopamine levels in the nucleus accumbens in response to externally defined stimulation of the VTA before and after each of three self-stimulation series lasting 10 min. Each series of self-stimulation was followed by decreases in the extracellular dopamine level, reflecting depletion of the intracellular dopamine pool during long-term self-stimulation of the VTA. These data lead to the conclusion that this method of teaching rats electrical self-stimulation in response to headlifts is promising for studying reinforcement mechanisms.
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References
Bychkov, E. R., Lebedev, A. A., Efimov, N. S., et al., “Features of the involvement of the dopaminergic and serotonergic systems of the brain in positive and negative emotional states in rats,” Obz. Klin. Farmakol. Lekarstv. Ter., 18, No. 2, 123–130 (2020).
Cheer, J. F., Wassum, K. M., and Heien, M. L., et al., “Cannabinoids enhance subsecond dopamine release in the nucleus accumbens of awake rats,” J. Neurosci., 24, No. 18, 4393–4400 (2004).
Droblenkov, A. V., Fedorov, A. V., and Shabanov, P. D., “Structural features of the dopaminergic nuclei of the ventral tegmentum of the midbrain,” Narkologiya, 17, No. 3, 41–45 (2018).
Fakhoury, M. and Rompré, P. P., “Intracranial self-stimulation and the curve-shift paradigm: a putative model to study the brain reward system,” in: The Brain Reward System, Fakhoury, M. (ed.), Humana, New York (2021), Vol. 165, pp. 3–20.
Fouriezos, G. and Randall, D., “The cost of delaying rewarding brain stimulation,” Behav. Brain Res., 87, No. 1, 111–113 (1997).
Ide, S., Takahashi, T., Takamatsu, Y., et al., “Distinct roles of opioid and dopamine systems in lateral hypothalamic intracranial self-stimulation,” Int. J. Neuropsychopharmacol., 20, No. 5, 403–409 (2017).
Lebedev, A. A., Bessolova, Yu. N., Efimov, N. S., et al., “Role of orexin peptide system in emotional overeating induced by brain reward stimulation in fed rats,” Res. Results Pharmacol., 6, No. 1, 81–91 (2020).
Lebedev, A. A., Rusanovskii, V. V., Lebedev, V. A., and Shabanov, P. D., Neurophysiology, Direkt-Media, Moscow, Berlin (2022).
Liebman, J. M., “Discriminating between reward and performance: a critical review of intracranial self-stimulation methodology,” Neurosci. Biobehav. Rev., 7, No. 1, 45–72 (1983).
Maiorov, V. I., “Functions of dopamine in the instrumental conditioned reflex,” Zh. Vyssh. Nerv. Deyat., 68, No. 4, 404–414 (2018).
Mena, S., Visentin, M., Witt, C. E., et al., “Novel, user-friendly experimental and analysis strategies for fast voltammetry: next generation FSCAV with artificial neural networks,” ACS Meas. Au, 2, No. 3, 241–250 (2022).
Mukhin, V. N., Borovets, I. R., Sizov, V. V., et al., “β-Amyloid and lithium influence the magnitude of phasic dopamine releases in the nucleus accumbens shell,” Zh. Vyssh. Nerv. Deyat., 70, No. 4, 488–499 (2020).
Negus, S. S. and Miller, L. L., “Intracranial self-stimulation to evaluate abuse potential of drugs,” Pharmacol. Rev., 66, No. 3, 869–917 (2014).
Pallikaras, V. and Shizgal, P., “Dopamine and beyond: implications of psychophysical studies of intracranial self-stimulation for the treatment of depression,” Brain Sci., 12, No. 8, 1052 (2022).
Panagis, G., Vlachou, S., Higuera-Matas, A., and Simon, M., “Editorial: neurobehavioral mechanisms of reward: theoretical and technical perspectives and their implications for psychopathology,” Front. Behav. Neurosci., 16, 967922 (2022).
Pavlov, I. P., Complete Works, Vol. 3, Book 1, Twenty Years of Experience in the Objective Study of Higher Nervous Activity (Behavior), USSR Academy of Sciences Press, Moscow (1951–1954), 2nd ed.
Paxinos, G. and Watson, C., The Rat Brain in Stereotaxic Coordinates, Elsevier Academic Press, San Diego (2005).
Petrov, E. S. and Lebedev, A. A., “Dopamine and the reinforcing system of the brain,” Neurosci. Behav. Physiol., 27, No. 3, 309–311 (1997).
Pyurveev, S. S., Sizov, V. V., Lebedev, A. A., et al., “Recording of changes in extracellular dopamine levels in the nucleus accumbens by fast-scanning cyclic voltammetry during stimulation of the ventral tegmental area, arousal of which induces a self-stimulation reaction,” Ros. Fiziol. Zh., 108, No. 10, 1316–1328 (2022).
Rodeberg, N. T., Johnson, J. A., Bucher, E. S., and Wightman, R. M., “Dopamine dynamics during continuous intracranial self-stimulation: effect of waveform on fast-scan cyclic voltammetry data,” ACS Chem. Neurosci, 7, No. 11, 1508–1518 (2016).
Rothman, R. B. and Baumann, M. H., “Monoamine transporters and psychostimulant drugs,” Eur. J. Pharmacol., 479, No. 1–3, 23–40 (2003).
Shabanov, P. D., Lebedev, A. A., and Meshcherov, Sh. K., Dopamine and the Reinforcing System of the Brain, Lan’, St. Petersburg (2002).
Simonov, P. V., The Emotional Brain, Nauka, Moscow (1980).
Solomon, R. B., Conover, K., and Shizgal, P., “Valuation of opportunity costs by rats working for rewarding electrical brain stimulation,” PLoS One, 12, No. 8, e0182120 (2017).
Trujillo-Pisanty, I., Conover, K., Solis, P., et al., “Dopamine neurons do not constitute an obligatory stage in the final common path for the evaluation and pursuit of brain stimulation reward,” PLoS One, 15, No. 6, e0226722 (2020).
Velazquez-Martinez, D. N., Pacheco-Gomez, B. L., Toscano-Zapien, A. L., et al., “On the similarity between the reinforcing and the discriminative properties of intracranial self-stimulation,” Front. Behav. Neurosci., 16, 799015 (2022).
Yavich, L. and Tiihonen, J., “Patterns of dopamine overflow in mouse nucleus accumbens during intracranial self-stimulation,” Neurosci. Lett., 293, No. 1, 41–44 (2000).
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Translated from Zhurnal Vysshei Nervnoi Deyatel’nosti imeni I. P. Pavlova, Vol. 73, No. 4, pp. 563–576, July–August, 2023.
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Sizov, V.V., Lebedev, A.A., Pyurveev, S.S. et al. A Method for Training Rats to Electrical Self-Stimulation in Response to Raising the Head Using a Telemetry Apparatus to Record Extracellular Dopamine Levels. Neurosci Behav Physi 54, 52–60 (2024). https://doi.org/10.1007/s11055-024-01568-z
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DOI: https://doi.org/10.1007/s11055-024-01568-z