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Von Willebrand Factor Activity in Rats after Transient Cerebral Ischemia

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One of the leading risk factors for recurrent cerebrovascular accidents after cerebral ischemia is dysfunction of the vascular endothelium. Von Willebrand factor (vWF) is a widely recognized marker for endothelial dysfunction associated with imbalance of the release of endothelial substances with procoagulant activity. vWF activity and the hematocrit were studied in Wistar rats after cerebral ischemia induced by 12-min occlusion of both carotid arteries with simultaneous hypotension (45 ± 2 mmHg). Postischemic changes were evaluated in five groups of rats: at 1 h and on days 3, 7, 14, and 21 after ischemia/reperfusion (I/R). Controls consisted of Wistar rats subjected to the same procedure but without ligation of the carotid arteries and hypotension. An increase in vWF activity was seen at 1 h after I/R. By day 3 of the postischemic period, the plasma vWF concentration decreased, which was evidenced by a decrease in the hematocrit to be due to hemodilution. There was a sharp increase in marker activity at postischemia day 7. vWF activity then decreased, reaching the level in control rats by day 21 after I/R. These data provide evidence that single 12-min episodes of I/R of the brain lead to the development of vascular endothelial dysfunction associated with imbalance in the release of endothelial substances with procoagulant activity, which persisted for 14 days after ischemia.

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Authors and Affiliations

  1. Pavlov Institute of Physiology, Russian Academy of Sciences, St. Petersburg, Russia

    V. N. Shuvaeva & O. P. Gorshkova

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  1. V. N. Shuvaeva
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Correspondence to V. N. Shuvaeva.

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Translated from Rossiiskii Fiziologicheskii Zhurnal imeni I. M. Sechenova, Vol. 106, No. 8, pp. 964–973, August, 2020.

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Shuvaeva, V.N., Gorshkova, O.P. Von Willebrand Factor Activity in Rats after Transient Cerebral Ischemia. Neurosci Behav Physi 51, 496–500 (2021). https://doi.org/10.1007/s11055-021-01096-0

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  • DOI: https://doi.org/10.1007/s11055-021-01096-0

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