Objective. To evaluate the dynamics of the formation of GABAergic neurons in the cortex and nuclei of the developing cerebellum of white rats during postnatal ontogeny using immunohistochemical detection of glutamate decarboxylase (GDC). Materials and methods. Studies were carried out on 16 mongrel white rats on days 2, 7, and 15 (the early postnatal period) and on day 45 (the pubertal period) after birth. GABAergic neurons were detected immunohistochemically using GAD67 primary mouse monoclonal antibodies to GDC, this being the enzyme synthesizing γ-aminobutyric acid. GDC immunoreactivity was studied on paraffin sections of the paravermal zone of the cerebellum. Results. GDC was detected in the cytoplasm of the bodies of all types of GABAergic neurons in the cerebellar cortex on postnatal day 2. The cytoplasm of the bodies of piriform (Purkinje) cells (PC) showed a maximum level on day 7, while their dendrites showed a maximum on day 15, and there was a subsequent reduction in large stellate cells (Golgi cells, GC) on day 45. GDC immunoreactivity in the axons of stellate and basket neurons was significantly elevated by day 45 and these formed plexuses (baskets) around PC bodies. GABAergic synapses were detected in the cerebellar cortex on day 7 and reached maximal development on day 45. The cytoplasm of the bodies of some neurons in the globose and emboliform nuclei of the cerebellum showed moderate GDC immunoreactivity in two-day-old rats. On day 7, GDC-immunopositive fi bers and axodendritic synapses were seen between neurons, with axosomatic synapses on neuron bodies, the number of these structures increasing by day 45. Conclusions. Immunohistochemical studies of GDC clearly identifi ed the dynamics of postnatal structural transformations of the bodies, processes, and synaptic terminals of GABAergic neurons in the rat cerebellum.
Similar content being viewed by others
References
D. E. Korzhevskii, O. V. Gilerovich, O. V. Kirik, et al., “Simultaneous detection of glutamate decarboxylase and synaptophysin in paraffin sections of the rat cerebellum,” Morfologiya, 147, No. 1, 74–77 (2015), doi: https://doi.org/10.1007/s11055-015-0205-6.
S. N. Olenev, The Developing Brain, Nauka, Leningrad (1978).
B. S. Sukhareva, E. L. Darii, and R. R. Khristoforov, “Glutamate decarboxylase: structure and catalytic properties,” Usp. Biol. Khim., No. 41, 131–162 (2001).
J. Altman, “Postnatal development of the cerebellar cortex in the rat. II. Phases in the maturation of Purkinje cells and of the molecular layer,” J. Comp. Neurol., 145, No. 4, 399–463 (1972), doi: https://doi.org/10.1002/cne.901450402.
J. Altman, “Postnatal development of the cerebellar cortex in the rat. III. Maturation of the components of the granular layer,” J. Comp. Neurol., 145, No. 4, 465–513 (1972), doi: 10.1002/.
I. Dean, S. J. Robertson, and F. A. Edwards, “Serotonin drives a novel GABAergic synaptic current recorded in rat cerebellar Рurkinje cells: a Lugaro cell to Purkinje cell synapse,” J. Neurosci., 23, No. 11, 4457–4469 (2003).
K. F. Greif, M. G. Erlander, N. K. Tillakaratne, et al., “Postnatal expression of glutamate decarboxylases in developing rat cerebellum,” Neurochem. Res., 16, No. 3, 235–242 (1991), doi: https://doi.org/10.1007/bf00966086.
D. L. Kaufman, C. R. Houser, and A. J. Tobin, “Two forms of the gamma-aminobutyric acid synthetic enzyme glutamate decarboxylase have distinct intraneuronal distributions and cofactor interactions,” J. Neurochem., 56, No. 2, 720–723 (1991).
D. F. Owens and A. R. Kriegstein, “Is there more to GABA than synaptic inhibition?” Nat. Rev. Neurosci., 3, No. 9, 715–727 (2002), doi: https://doi.org/10.1038/nrn919.
G. Paxinos and C. Watson, The Rat Brain in Stereotaxic Coordinates, Academic Press, San Diego (2013), 7th ed.
K. Schilling, J. Oberdick, F. Rossi, et al., “Besides Purkinje cells and granule neurons: an appraisal of the cell biology of the interneurons of the cerebellar cortex,” Histochem. Cell Biol., 130, No. 4, 601–615 (2008), doi: https://doi.org/10.1007/s00418-008-0483-y.
M. Uusisaari and T. Knöpfel, “Functional classifi cation of neurons in the mouse lateral cerebellar nuclei,” Cerebellum, 10, No. 4, 637–646 (2011), doi: https://doi.org/10.1007/s12311010-0240-3.
M. D. Willcutts and M. Morrison-Bogorad, “Quantitative in situ hybridization analysis of glutamic acid decarboxylase messenger RNA in developing rat cerebellum,” Brain Res. Dev. Brain Res., 63, No. 1–2, 253–264 (1991), doi: https://doi.org/10.1016/0165-3806(91)90085-w.
L. Zhang and J. E. Goldman, “Developmental fates and migratory pathways of dividing progenitors in the postnatal rat cerebellum,” J. Comp. Neurol., 370, No. 4, 536–550 (1996), doi: https://doi.org/10.1002/(SICI)1096-9861(19960708)370:4<536::AID-CNE9>3.0;CO;2-5.
L. Zhang and J. E. Goldman, “Generation of cerebellar interneurons from dividing progenitors in white matter,” Neuron, 16, No. 1, 47–54 (1996), doi: https://doi.org/10.1016/s0896-6273(00)80022-7.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Morfologiya, 157, No. 1, pp. 13–17, January–February, 2020.
Rights and permissions
About this article
Cite this article
Zimatkin, S.M., Karniushko, O.A. Postnatal Development of GABAergic Neurons in the Rat Cerebellum. Neurosci Behav Physi 50, 957–960 (2020). https://doi.org/10.1007/s11055-020-00990-3
Received:
Revised:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11055-020-00990-3