Abstract
The present work investigated the possibility of preparing nanoparticles based on methacryloylglycylglycine (MAGG) and chitosan (CS) by in situ polymerization. The study revealed that nanoparticle formation was strictly dependent on ionic interactions between NH3 + groups from CS and COO− groups arising from the anionic monomer MAGG. The subsequent in situ polymerizations of MAGG in the presence of CS led to the formation of nanoparticles with homogeneous morphology, a uniform particle size distribution, and a good spherical shape as confirmed by laser diffraction granulometry and scanning electron microscopy analyses. Nanoparticle formulations with different amounts of CS and MAGG were prepared, and their chemical compositions were investigated by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The obtained results showed that the polymerization of MAGG in the presence of CS appears to be a very promising approach in the preparation of nanoparticles for drug delivery applications.
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Assaad E, Wang YJ, Zhu XX, Mateescu MA (2011) Polyelectrolyte complex of carboxymethyl starch and chitosan as drug carrier for oral administration. Carbohydr Polym 84:1399–1407
Baier J, Koetz J, Kosmella S, Tiersch B, Rehage HJ (2007) Polyelectrolyte-Modified Inverse microemulsions and their use as templates for the formation of magnetite nanoparticles. Phys Chem B 111:8612–8618
Chatterjee A, Zhao L, Zhang L, Pradhan D, Zhou X, Leung KT (2008) Core-level electronic structure of solid-phase glycine, glycyl-glycine, diglycyl-glycine, and polyglycine: X-ray photoemission analysis and Hartree-Fock calculations of their zwitterions. J Chem Phys 129:105104
Chiellini F, Bartoli C, Dinucci D, Piras AM, Anderson R, Croucher T (2007) Bioeliminable polymeric nanoparticles for proteic drug delivery. Int J Pharm 343:90–97
Chiellini F, Piras AM, Errico C, Chiellini E (2008) Micro/nanostructured polymer systems for biomedical and pharmaceutical applications. Nanomedicine 3:367–398
Cometa S, Chiellini F, Bartolozzi I, Chiellini E, De Giglio E, Sabbatini L (2010) Surface segregation assessment in poly(7-caprolactone)-poly(ethylene glycol) multiblock copolymer films. Macromol Biosci 10:317–327
Cometa S, Iatta R, Ricci MA, Ferretti C, De Giglio E (2013) Analytical characterization and antimicrobial properties of novel copper nanoparticles-loaded electrosynthesised hydrogel coatings. J Bioact Compat Polym 28:508–522
Dash M, Ferri M, Chiellini F (2012) Synthesis and characterization of semi-interpenetrating polymer network hydrogel based on chitosan and poly(methacryloilglycilglycine). Mater Chem Phys 135:1070–1076
De Moura MR, Aouada FA, Mattoso LHC (2008) Preparation of chitosan nanoparticles using methacrylic acid. J Colloid Interface Sci 321:477–483
De Vasconcelos CL, Bezerril PM, Dos Santos DES, Dantas TNC, Pereira MR, Fonseca JLC (2006) Effect of molecular weight and ionic strength on the formation of polyelectrolyte complexes based on poly(methacrylic acid) and chitosan. Biomacromolecules 7:1245–1252
Fenga C, Wangb Z, Jiangc C, Konga M, Lia Y, Chenga X, Chena X (2013) Chitosan/o-carboxymethyl chitosan nanoparticles for efficient and safe oral anticancer drug delivery: in vitro and in vivo evaluation. Int J Pharm 457:158–167
Gan Q, Wang T (2007) Chitosan nanoparticle as protein delivery carrier—systematic examination of fabrication conditions for efficient loading and release. Colloids Surf B 59:24–34
Gujarathi NA, Rane BR, Patel JK (2013) pH sensitive polyelectrolyte complex of O-carboxymethyl chitosan and poly (acrylic acid) cross-linked with calcium for sustained delivery of acid susceptible drugs. Int J Pharm 436:418–425
Hamman JH (2010) Chitosan based polyelectrolyte complexes as potential carrier materials in drug delivery systems. Mar Drugs 8:305–322
Hellmers F, Ferguson P, Krull R, Margaritas A (2013) Characterization and in vitro cytotoxicity of doxorubicin-loaded γ-polyglutamic acid-chitosan composite nanoparticles. Biochem Eng J 75:72–78
Hu Y, Ding Y, Ding D, Sun M, Zhang L, Jiang X, Yang C (2007) Hollow chitosan/poly(acrylic acid) nanospheres as dry carriers. Biomacromolecules 8:1069–1076
Huang Y, Nan A, Rosen GM, Winalski CS, Schneider E, Ghandehari H (2003) N-(2-hydroxypropyl)methacrylamide (HPMA) copolymer-linked nitroxides: potential magnetic resonance contrast agents. Macromol Biosci 3:647–652
Kumari A, Yadav SK, Yadav SC (2010) Biodegradable polymeric nanoparticles based drug delivery systems. Colloids Surf B 75:1–18
Liu P, Zhao X (2013) Facile preparation of well-defined near-monodisperse chitosan/sodium alginate polyelectrolyte complex nanoparticles (CS/SAL NPs) via ionotropic gelification: a suitable technique for drug delivery systems. Biotechnol J 8:847–854
Rampino A, Borgogna M, Blasi P, Bellich B, Cesàro A (2013) Chitosan nanoparticles: preparation, size evolution and stability. Int J Pharm 455:219–228
Vasir JK, Labhasetwar V (2005) Targeted drug delivery in cancer therapy. Technol Cancer Res Treat 4:363–374
Vinagradov SV, Bronich TK, Kabanov AV (2002) Nanosized cationic hydrogels for drug delivery: preparation, properties and interactions with cells. Adv Drug Deliv Rev 54:223–233
Wang H, Li W, Lu Y, Wang Z (1997) Studies on Chitosan and poly(acrylic acid) interpolymer complex. I. Preparation, structure, pH-sensitivity, and salt sensitivity of complex-forming poly(acrylic acid): chitosan semi-interpenetrating polymer network. J Appl Polym Sci 65:1445–1450
Winkelmann M, Gold J, Hauert R, Kasemo C, Spencer ND, Brunette DM, Textor M (2003) Chemically patterned, metal oxide based surfaces produced by photolithographic techniques for studying protein– and cell–surface interactions I: microfabrication and surface characterization. Biomaterials 24:1133–1145
Yi H, Wu LQ, Bentley WE, Ghodssi R, Rubloff GW, Culver JN (2005) Biofabrication with chitosan. Biomacromolecules 6:2881–2894
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Marcella Ferri and Mamoni Dash contributed equally to the work.
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Ferri, M., Dash, M., Cometa, S. et al. Preparation and characterization of hybrid nanoparticles based on chitosan and poly(methacryloylglycylglycine). J Nanopart Res 16, 2422 (2014). https://doi.org/10.1007/s11051-014-2422-2
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DOI: https://doi.org/10.1007/s11051-014-2422-2