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Genome-wide characterization and expression analysis of the HD-Zip II gene family in response to drought and GA3 stresses in Nicotiana tabacum

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Abstract

Background

Homeodomain-leucine ZIPper (HD-ZIP) transcription factors play crucial roles in plant growth, development, and stress responses. The HD-ZIP family is categorised into four groups (HD-ZIP I-IV). While extensive genome-wide studies have been conducted on the HD-ZIP I, III, and IV subfamily in Nicotiana tabacum (tobacco), comprehensive reports on the HD-ZIP II subfamily genes are limited.

Methods

Bioinformatics resources and tools were utilised to analyse molecular characteristics, phylogenetic homology, and protein interactions. Expression pattern analyses in various tissues and the relative expression of NtHD-ZIP II genes under drought and GA3 treatment were assessed by qRT-PCR.

Results

In this study, 24 HD-ZIP II members were systematically identified and categorised into seven independent clades through phylogenetic analysis involving tobacco and other plant species. We found that 19 NtHD-ZIP II genes exhibited tissue-specific expression. The transcripts of NtHD-ZIPII3, 4, 14, 23, 24 were notably induced under the drought treatments, while those of NtHD-ZIPII7, 11, 12, 20 were suppressed. Furthermore, NtHD-ZIPII15 transcripts decreased following GA3 treatment, whereas the transcripts of NtHD-ZIPII7, 8, 11, 12 were induced after GA3 treatment. Notably, an increase in trichomes was observed in tobacco leaves treated with GA3 and subjected to drought.

Conclusions

The expression levels of some HD-ZIP II genes were altered, and an increase in glandular trichomes was induced under GA3 and drought treatments in tobacco. Overall, our findings provide insights into the expression patterns of NtHD-ZIP II genes and will facilitate their functional characterisation in future studies.

Highlights

Genome-Wide Identification and Expression Analysis of 24 HD-ZIP II Subfamily Genes in Nicotiana tabacum Including, Differential Expression in Leaves Under Drought and GA3 Stress as Assessed by qRT-PCR.

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Data availability

No Data associated in the manuscript.

Abbreviations

HD-ZIP:

Homeodomain-leucine ZIPper

Nt:

Nicotiana tabacum

CPSCE:

Cys, Pro, Ser, Cys, Glu

START:

steroidogenic acute regulatory protein-lipid transfer domain

SAD:

START associated domain

MEKHLA:

Met-Glu-Lys-His-Leu-Ala domain

WT:

wild type

HOX:

homeobox

UTR:

untranslated region

CDS:

Coding sequence

GA:

gibberellin

ABA:

abscisic acid

JA:

jasmonic acid

SA:

salicylic acid

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Funding

Engineering Technology Research Center for The Processing of Pepper Products of Guizhou [Qianjiaohe KV (2021) 006], Engineering and Technology Research Center for Pepper Fermented Products of Guizhou [Qiankehe Platform Talent (2020) 2102], Guizhou Provincial Science and Technology Projects [Qian Ke He Zhi Cheng, no. (2021) 262, (2022) 172, (2022) 156, (2023) 455].

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Authors and Affiliations

  1. Key laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang, 550025, Guizhou Province, China

    Liu Liu, Lincheng Zhang, Longhuan Yang, Jiahua Zheng, Jing Jin, Shuoqiu Tong & Yongjun Wu

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  1. Liu Liu
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Contributions

All authors contributed to the study conception and design. Yongjun Wu get funding acquisition for the financial support from funding agencies. Material preparation, data collection and analysis were performed by Lincheng Zhang, Liu Liu, Longhuan Yang and Jiahua Zheng. The first draft of the manuscript was written by Liu Liu and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Yongjun Wu.

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Liu, L., Zhang, L., Yang, L. et al. Genome-wide characterization and expression analysis of the HD-Zip II gene family in response to drought and GA3 stresses in Nicotiana tabacum. Mol Biol Rep 51, 581 (2024). https://doi.org/10.1007/s11033-024-09527-0

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