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Genetic enhancement of reproductive stage drought tolerance in RPHR-1005R and derivative rice hybrids through marker-assisted backcross breeding in rice (Oryza sativa L.)

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Abstract

Background

Drought stress is considered as one of the major production constraints in rice. RPHR-1005R is a restorer line (R-Line) with a popular, medium-slender grain type, and is the male parent of the popular Indian rice hybrid, DRR-H3. However, both the hybrid and its restorer are highly vulnerable to the drought stress, which limits the adoption of the hybrid. Therefore, the selection of the restorer line RPHR-1005R has been made with the objective of enhancing drought tolerance.

Methods and results

In this study, we have introgressed a major QTL for grain yield under drought (qDTY 1.1) from Nagina22 through a marker-assisted backcross breeding (MABB) strategy. PCR based SSR markers linked to grain yield under drought (qDTY1.1 - RM431, RM11943), fertility restorer genes (Rf3-DRRM-Rf3-10, Rf4-RM6100) and wide compatibility (S5n allele) were deployed for foreground selection. At BC2F1, a single plant (RPHR6339-4-16-14) with target QTL in heterozygous condition and with the highest recurrent parent genome recovery (85.41%) and phenotypically like RPHR-1005R was identified and selfed to generate BC2F2. Fifty-eight homozygous lines were advanced to BC2F4 and six promising restorer lines and a hybrid combination (APMS6A/RPHR6339-4-16-14-3) were identified.

Conclusions

In summary, the six improved restorer lines could be employed for developing heterotic hybrids possessing reproductive stage drought tolerance. The hybrid combination (APMS6A/RPHR6339-4-16-14-3) was estimated to ensure stable yields in drought-prone irrigated lowlands as well as in directly seeded aerobic and upland areas of India.

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Data Availability

All data supporting the findings of this study are available within the paper and its Supplementary Information. It is also available from the corresponding author upon reasonable request.

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Funding

This work was supported by ICAR-Indian Institute of Rice Research (ICAR-IIRR), National Innovations on Climate Resilient Agriculture (NICRA) and Govt. of India (F. No. Phy/NICRA/2011–2019). Author Senguttuvel P has received research support from ICAR-IIRR.

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  1. Crop Improvement Section, ICAR-Indian Institute of Rice Research, Hyderabad, 500030, India

    N. Sravanraju, P. Beulah, V. Jaldhani, P. Nagaraju, A. S. HariPrasad, P. Brajendra, R. M. Sundaram & P. Senguttuvel

  2. Biotechnology Department, Jawaharlal Nehru Technological University (JNTU-H), Hyderabad, 500085, India

    N. Sravanraju & N. Sunitha

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  1. N. Sravanraju
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Contributions

NS and PS contributed to the study conception and design. Material preparation, data collection and analysis were performed by NS, PB, VJ and PN. The first draft of the manuscript was written by NS and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to R. M. Sundaram or P. Senguttuvel.

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Sravanraju, N., Beulah, P., Jaldhani, V. et al. Genetic enhancement of reproductive stage drought tolerance in RPHR-1005R and derivative rice hybrids through marker-assisted backcross breeding in rice (Oryza sativa L.). Mol Biol Rep 51, 426 (2024). https://doi.org/10.1007/s11033-024-09351-6

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