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Molecular cloning and gene expression of acc2 from grass carp (Ctenopharyngodon idella) and the regulation of glucose metabolism by ACCs inhibitor

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Abstract

Background

Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA. Malonyl-CoA, which plays a key role in regulating glucose and lipid metabolism, is not only a substrate for fatty acid synthesis but also an inhibitor of the oxidation pathway. ACC exists as two isoenzymes that are encoded by two different genes. ACC1 in grass carp (Ctenopharyngodon idellus) has been cloned and sequenced. However, studies on the cloning, tissue distribution, and function of ACC2 in grass carp were still rare.

Methods and results

The full-length cDNA of acc2 was 8537 bp with a 7146 bp open reading frame encoding 2381 amino acids. ACC2 had a calculated molecular weight of 268.209 kDa and an isoelectric point of 5.85. ACC2 of the grass carp shared the closest relationship with that of the common carp (Sinocyclocheilus grahami). The expressions of acc1 and acc2 mRNA were detected in all examined tissues.  The expression level of acc1 was high in the brain and fat but absent in the midgut and hindgut. The expression level of acc2 in the kidney was significantly higher than in other tissues, followed by the heart, brain, muscle, and spleen. ACCs inhibitor significantly reduced the levels of glucose, malonyl-CoA, and triglyceride in hepatocytes.

Conclusions

This study showed that the function of ACC2 was evolutionarily conserved from fish to mammals. ACCs inhibitor inhibited the biological activity of ACCs, and reduced fat accumulation in grass carp.

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Data availability

All data are available from the corresponding author by request.

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Funding

This study was supported by the National Natural Science Foundation of China (No.31702361).

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Author notes

  1. Manxia Cao and Xinyuan Li are equally the first authors.

Authors and Affiliations

  1. Key Laboratory for Animal Nutrition and Feed Science of Hubei Province, Wuhan Polytechnic University, Wuhan, 430023, China

    Manxia Cao, Xinyuan Li & Feng Huang

  2. Key Laboratory of Freshwater Biodiversity Conservation, Yangtze River Fisheries Research Institute, The Ministry of Agriculture and Rural Affairs, Chinese Academy of Fishery Sciences, No. 8, Wudayuan 1st Road, Donghu Hi-tech Development Zone, Wuhan, 430223, China

    Manxia Cao, Xinyuan Li, Lixue Dong, Hua Wen, Ming Jiang, Xing Lu & Juan Tian

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  1. Manxia Cao
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Contributions

Juan Tian and Feng Huang designed the research; Manxia Cao, Xinyuan Li, Jianmin Zhang, Lixue Dong, Ming Jiang, Hua Wen, and Xing Lu conducted the experiments and analyzed the data; Manxia Cao, Xinyuan Li, and Juan Tian wrote the paper.

Corresponding authors

Correspondence to Feng Huang or Juan Tian.

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Competing interests

The authors declare no competing interests.

Ethics approval

Grass carp (Ctenopharyngodon idella) is widely cultivated in China and are not listed as endangered or protected species. All animal care and use procedures were approved by the Institutional Animal Care and Use Committee of Yangtze River Fisheries Research Institute (according to YFI 2018-40 of July 20, 2018). Grass carp were anesthetized with MS-222 in 80 mg L−1 water to minimum suffering before being assigned to tanks and were anesthetized death with MS-222 in 80 mg L−1 water when sampling muscle and hepatopancreas in this experiment.

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Cao, M., Li, X., Dong, L. et al. Molecular cloning and gene expression of acc2 from grass carp (Ctenopharyngodon idella) and the regulation of glucose metabolism by ACCs inhibitor. Mol Biol Rep 51, 402 (2024). https://doi.org/10.1007/s11033-024-09286-y

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