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Occurrence and characterization of Proteus mirabilis from infected farmed African catfish in Ogun State, Nigeria

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Abstract

Background

Bacterial pathogens are the causative agents of some of the most serious disease problems in cultured fish causing mortalities and severe economic losses. This study was conducted to determine the occurrence and characterization of Proteus mirabilis from infected farmed African catfish in Ogun State, Nigeria.

Methodology

The bacteria were isolated from diseased farmed African catfish (Clarias gariepinus, n=128) with clinical signs of skin haemorrhages, ulceration, and ascites purposively sampled from farms within three senatorial districts namely Ogun East (OE; n=76), Ogun Central (OC; n=30) and Ogun West (OW; n=22) in Ogun State. The isolates were identified based on morphological characteristics, biochemical tests, and 16S rRNA gene characterisation. The 16S rRNA gene sequences were analysed using BLAST, submitted to the NCBI database, and an accession number was generated.

Results

The occurrence of Proteus mirabilis in infected Clarias gariepinus was 13.16%, 25%, and 31.25% in OE, OC, and OW, respectively. A significantly higher incidence was recorded in OW compared to other areas. All the Proteus mirabilis isolates were motile, gram-negative, short rod, non-lactose fermenter bacteria that showed positive catalase reactions, negative oxidase, and positive for methyl-red. The Proteus mirabilis isolates (OP 594726.1) were closely related to isolates from Pakistan, Italy, and India

Conclusions

We conclude that Proteus mirabilis colonises farmed Clarias gariepinus in Ogun State, Nigeria and the identified strain showed an evolutionary relationship with known pathogenic NCBI reference strains from other countries.

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Data availability

The datasets used and or analysed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

We want to acknowledge Miss Nike Dada for her assistance during Bioinformatics.

Funding

This study was completely funded by the personal funds of the authors.

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Authors and Affiliations

  1. Department of Veterinary Medicine, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Nigeria

    Olayinka Remilekun Anifowose, Gbolahanmi Akinola Oladosu & Oladipo Olufemi Omotosho

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  1. Olayinka Remilekun Anifowose
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  2. Gbolahanmi Akinola Oladosu
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  3. Oladipo Olufemi Omotosho
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Contributions

OR and GA Conceptualized the study, OR, GA, and OO generated data and carried out fieldwork, OR analyzed data and made the manuscript draft, GA and OO edited the manuscript, OR, GA, and OO approved the final manuscript.OR and GA Conceptualized the study, OR, GA, and OO generated data and carried out fieldwork, OR analyzed data and made the manuscript draft, GA and OO edited the manuscript, OR, GA, and OO approved the final manuscript.OR and GA Conceptualized the study, OR, GA, and OO generated data and carried out fieldwork, OR analyzed data and made the manuscript draft, GA and OO edited the manuscript, OR, GA, and OO approved the final manuscript.OR and GA Conceptualized the study, OR, GA, and OO generated data and carried out fieldwork, OR analyzed data and made the manuscript draft, GA and OO edited the manuscript, OR, GA, and OO approved the final manuscript.

Corresponding author

Correspondence to Olayinka Remilekun Anifowose.

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The authors declare no competing interests.

Ethical approval

Ethical approval was received from the University of Ibadan, Animal Care and Use Research Ethics Committee (UI-ACUREC) with the assigned number UI-ACUREC/056–0622/9.

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Anifowose, O.R., Oladosu, G.A. & Omotosho, O.O. Occurrence and characterization of Proteus mirabilis from infected farmed African catfish in Ogun State, Nigeria. Mol Biol Rep 51, 446 (2024). https://doi.org/10.1007/s11033-023-08973-6

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