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Fucoidan from Sargassum wightii reduces oxidative stress through upregulating Nrf2/HO-1 signaling pathway in alloxan-induced diabetic cardiomyopathy rats

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Abstract

Background

Diabetic cardiomyopathy (DCM) is a form of cardiac dysfunction caused by diabetes, increasing heart failure and death. Studies shown that hyperglycemia-induced oxidative stress significantly affects heart structure and functional changes during diabetic cardiomyopathy. Fucoidans are sulfated polysaccharide derived from naturally available seaweeds and reported for various biological functions such as antioxidant, anti-diabetic, and anti-inflammatory. However, the therapeutic potential of Indian seaweeds against DCM remains largely unexplored. Therefore, the current study aimed to work on the cardioprotective effect of extracted fucoidan from Sargassum wightii (SwF) in alloxan-induced DCM.

Methods and results

Diabetes (DM) was induced with alloxan monohydrate (150 mg/kg−1) dissolved in Nacl (0.9%) overnight–fasted rats. Group III, IV rats were DM induced, followed by treated with SwF (150 mg/kg−1) and (300 mg/kg−1). Group V and VI were non-diabetic rats and received SwF (150 mg/kg−1) and (300 mg/kg−1). SwF reduced classical progressive DM complications such as hyperglycemia, polydipsia, polyphagia, and polyurea in alloxan-induced diabetic rats. Biochemical analysis showed that SwF decreased blood glucose, cardiac markers enzymes, and lipid peroxidation levels compared to diabetic rats. SwF administration significantly increased Nrf2, HO-1, SOD, Catalase, and NQO1 gene expression. In addition, SwF-treated rats showed reduced heart tissue damage with increased Nrf2 and HO-1 protein expression.

Conclusion

The current research concludes that targeting oxidative stress with SwF provided an effective role in the prevention of DCM. Thus, fucoidan could be used to develop functional food ingredients for DCM.

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Data availability

The authors declare that all data supporting the findings of this study are available within the article and its supplementary information files.

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Acknowledgements

Shanavas Syed Mohamed Puhari expresses his gratitude to the UGC BSR fellowship program, New Delhi, India. The authors also thank, UGC-CEGS, UGC-CAS, UGC-NRCBS, DST PURSE and DST-FIST program for the central instrumentation facility at SBS, MKU.

Funding

The authors declare that no grants were received during the preparation of this manuscript.

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Authors and Affiliations

  1. Molecular Cardiology Unit, Department of Biochemistry, Centre for Excellence in Genomic Sciences, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, 625021, India

    Shanavas Syed Mohamed Puhari, Subramani Yuvaraj, Varadaraj Vasudevan & Govindan Sadasivam Selvam

  2. Center for Molecular and Translational Medicine, Georgia State University, Atlanta, GA, USA

    Tharmarajan Ramprasath

  3. Department of Plant Science, School of Biological Sciences, Central University of Kerala, Periye, Kasaragod, Kerala, 671320, India

    Kulanthaiyesu Arunkumar

  4. Department of Animal behaviour & Physiology, School of Biological Sciences, Madurai Kamaraj University, Madurai, Tamil Nadu, India

    Chinnaiah Amutha

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Contributions

Conceptualization—SSMP, GSS; Methodology—SSMP, SY, VV; Formal analysis—SSMP, TR, GSS; Investigation—SSMP, SY, VV; Writing - Original draft – SSMP, SY; Review and Editing – SSMP, SY, VV, TR, KA, CA and GSS; Funding acquisition – GSS.

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Correspondence to Govindan Sadasivam Selvam.

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The experimental procedure was approved by the Internal Research and Review Board, Ethical Clearance, Biosafety and Animal Welfare Committee of Madurai Kamaraj University.

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Puhari, S.S.M., Yuvaraj, S., Vasudevan, V. et al. Fucoidan from Sargassum wightii reduces oxidative stress through upregulating Nrf2/HO-1 signaling pathway in alloxan-induced diabetic cardiomyopathy rats. Mol Biol Rep 50, 8855–8866 (2023). https://doi.org/10.1007/s11033-023-08780-z

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