Abstract
Background
The incidence rate of ovarian carcinoma (OC) is the third of the female reproductive system malignant tumors, while its mortality rate ranks first among causes of female reproductive system tumor related death in the world.
Methods
In the present research, we investigated the specific role of LIMD2 through LIMD2 knockdown in OC cells.
Results
The results of online analysis and expression detection proved that LIMD2 was up-regulated in human OC tissues and cells. Knockdown of LIMD2 inhibited the proliferation, migration and invasion in OC cells. LIMD2 knockdown promoted the apoptosis, as well as the expression of Cleaved-Caspase3 and Bax. Importantly, knockdown of LIMD2 promotes cell autophagy. LC3-II/I ratio and Beclin1 expression increased in LIMD2 knockdown cells, while P62 expression declined in LIMD2 knockdown cells. Additionally, the phosphorylation of ERK1/2 was inhibited by the knockdown of LIMD2 in SKOV3 and OVCAR3 cells.
Conclusion
Knockdown of LIMD2 inhibits cell proliferation, migration, invasion and autophagy, and promotes the apoptosis through the ERK1/2 signaling pathway, suggesting that LIMD2-siRNA may be an effective molecule to prevent OC progression.
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Data Availability
The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Funding
This study was supported by Research Fund of New Medical Clinical Translation Workstation of Academician He Lin of Jining Medical College, Grant/Award Number: JYHL2019FMS15.
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Haiyang Hu has made substantial contributions to the conception and design of the work. Yanan Wang performed the experiments and wrote the paper. Yan Dong and Lin Wang assisted with the experiments, Yahui Chen and Yan Zhou assisted with the data analysis. Lin Sun helped analyzed the data and modify the paper. Each author has made substantial contributions to the acquisition, analysis, and interpretation of data.
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Hu, H., Wang, Y., Dong, Y. et al. Knockdown of LIMD2 inhibits the progression of ovarian carcinoma through ERK1/2 pathway. Mol Biol Rep 50, 8985–8993 (2023). https://doi.org/10.1007/s11033-023-08733-6
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DOI: https://doi.org/10.1007/s11033-023-08733-6