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Gene expression study of host-human T-cell leukaemia virus type 1 (HTLV-1) interactions: adult T-cell leukaemia/lymphoma (ATLL)

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Abstract

Background

In HTLV-1-associated malignant disease, adult T-cell leukaemia/lymphoma (ATLL), the interaction of virus and host was evaluated at the chemokines gene expression level. Also, IL-1β and Caspase-1 expressions were evaluated to investigate the importance of pyroptosis in disease development and progression.

Methods and results

The expression of host CCR6 and CXCR-3 and the HTLV-1 proviral load (PVL), Tax, and HBZ were assessed in 17 HTLV-1 asymptomatic carriers (ACs) and 12 ATLL patients using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR), TaqMan method. Moreover, RT-qPCR, SYBR Green assay were performed to measure Caspase-1 and IL-1β expression. HTLV-1-Tax did not express in 91.5% of the ATLLs, while HBZ was expressed in all ATLLs. The expression of CXCR3 dramatically decreased in ATLLs compared to ACs (p = 0.001). The expression of CCR6 was lower in ATLLs than ACs (p = 0.04). The mean of PVL in ATLL patients was statistically higher than ACs (p = 0.001). Furthermore, the expression of the IL-1β between ATLLs and ACs was not statistically significant (p = 0.4). In contrast, there was a meaningful difference between Caspase-1 in ATLLs and ACs (p = 0.02).

Conclusions

The present study indicated that in the first stage of ATLL malignancy toward acute lymphomatous, CXCR3 and its progression phase may target the pyroptosis process. Mainly, HBZ expression could be a novel therapeutic target.

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Data Availability

All data supporting this study’s findings are included in the manuscript and available from the corresponding author upon reasonable request.

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Acknowledgements

The results described in this paper were part of the student thesis. This study was subjected to MSc thesis in Medical Hematology and Medical Virology by M. Rahimzada and M. Nahavandi, respectively. The authors greatly thank the Vice-Chancellor for Research and Technology, Mashhad University of Medical Sciences, Mashhad, Iran, for financially supporting the present study. We also are grateful to our kind colleagues in Immunology Research Center, Inflammation and Inflammatory Diseases Division, for their valuable help and great appreciation to the ATLL patients and ACs participating in this study.

Funding

This study was financially supported by the Vice-Chancellor for Research and Technology, Mashhad University of Medical Sciences, Mashhad, Iran, under Grants [MUMS 971184 and MUMS 981133, recipient: SAR. Rezaee].

Author information

Authors and Affiliations

  1. Immunology Research Center, Inflammation and Inflammatory Diseases Division, Faculty of Medicine, Mashhad University of Medical Sciences, Azadi-Square, Medical Campus, Mashhad, 9177948564, Iran

    Masooma Rahimzada, Mona Saffari, Nazila Ariaee, Narges Valizadeh & Seyed Abdolrahim Rezaee

  2. Antimicrobial Resistance Research Center, Bu-Ali Research Institute, Mashhad University of Medical Sciences, Mashhad, Iran

    Mehri Nahavandi & Mohammad Derakhshan

  3. Department of Microbiology and Virology, Faculty of Medicine, Mashhad University of Medical Sciences, Azadi-Square, Medical Campus, Mashhad, 9177948564, Iran

    Mehri Nahavandi & Mohammad Derakhshan

  4. Blood Borne Infections Research Center, Academic Center for Education, Culture, and Research (ACECR), Razavi Khorasan, Mashhad, Iran

    Azam Shafaei, Arman Mosavat & Sanaz Ahmadi Gezeldasht

  5. Hematology and Oncology Division, Department of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Hossein Rahimi

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  1. Masooma Rahimzada
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Contributions

Doing experiments: MR, MN and MS; Manuscript drafting and editing: AS, AM, NA and SAG; Research advisors: HS and NV; Research director, conception and design of the study; data analysis: SAR and MD. All authors have read and approved the final manuscript.

Corresponding authors

Correspondence to Seyed Abdolrahim Rezaee or Mohammad Derakhshan.

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Competing interests

The authors declare no conflicts of interest regarding this manuscript in other regions.

Ethics approval/Consent to participate

This study was performed in line with the principles of the Declaration of Helsinki. The study was reviewed and approved by the Biomedical Research Ethics Committee of the Mashhad University of Medical Sciences [IR.MUMS.REC.971184 and IR.MUMS.REC.981133]. The written informed consent forms were obtained and signed by all the participants. All methods were performed following relevant guidelines and regulations.

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Rahimzada, M., Nahavandi, M., Saffari, M. et al. Gene expression study of host-human T-cell leukaemia virus type 1 (HTLV-1) interactions: adult T-cell leukaemia/lymphoma (ATLL). Mol Biol Rep 50, 7479–7487 (2023). https://doi.org/10.1007/s11033-023-08626-8

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